Establishment of an Efficient Sugarcane Transformation System via Herbicide-Resistant CP4-EPSPS Gene Selection

文献类型： 外文期刊

第一作者： Wang, Wenzhi

作者： Wang, Wenzhi;Javed, Talha;Shen, Linbo;Sun, Tingting;Yang, Benpeng;Zhang, Shuzhen;Wang, Wenzhi;Javed, Talha;Shen, Linbo;Sun, Tingting;Yang, Benpeng;Zhang, Shuzhen;Wang, Wenzhi;Wang, Wenzhi;Zhang, Shuzhen

作者机构：

关键词： sugarcane; Agrobacterium-mediated transformation; transgenic technology; herbicide resistance

期刊名称：PLANTS-BASEL （ 影响因子：4.5； 五年影响因子：4.8 ）

ISSN： 2223-7747

年卷期： 2024 年 13 卷 6 期

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收录情况： SCI

摘要： Sugarcane (Saccharum spp.), a major cash crop that is an important source of sugar and bioethanol, is strongly influenced by the impacts of biotic and abiotic stresses. The intricate polyploid and aneuploid genome of sugarcane has shown various limits for conventional breeding strategies. Nonetheless, biotechnological engineering currently offers the best chance of introducing commercially significant agronomic features. In this study, an efficient Agrobacterium-mediated transformation system that uses the herbicide-resistant CP4-EPSPS gene as a selection marker was developed. Notably, all of the plants that were identified by PCR as transformants showed significant herbicide resistance. Additionally, this transformation protocol also highlighted: (i) the high yield of transgenic lines from calli (each gram of calli generated six transgenic lines); (ii) improved selection; and (iii) a higher transformation efficiency. This protocol provides a reliable tool for a routine procedure for the generation of resilient sugarcane plants.

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