Description of two nitrogen-fixing bacteria, Geomonas fuzhouensis sp. nov. and Geomonas agri sp. nov., isolated from paddy soils

文献类型： 外文期刊

第一作者： Yang, Shang

作者： Yang, Shang;Tang, Rong;Han, Shuang;Xie, Cheng-Jie;Zhou, Shun-Gui;Yang, Shang;Liu, Guo-Hong;Tang, Rong;Han, Shuang;Xie, Cheng-Jie

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关键词： Geomonas fuzhouensis; Geomonas agri; Paddy soil; Nitrogen fixation

期刊名称：ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY （ 影响因子：2.158； 五年影响因子：2.745 ）

ISSN： 0003-6072

年卷期： 2022 年 115 卷 3 期

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收录情况： SCI

摘要： Two strictly anaerobic nitrogen-fixing strains, designated RG17(T) and RG53(T), were isolated from paddy soils in China. Strains RG17(T) and RG53(T) showed the highest 16S rRNA gene sequence similarities to the type strain Geomonas paludis (97.9-98.4%). Phylogenetic tree based on 16S rRNA gene sequences showed that two strains clustered with members of the genus Geomonas. Growth of strain RG17(T) was observed at 20-42 degrees C, pH 5.5-8.5 and 0-0.3% (w/v) NaCl while strain RG53(T) growth was observed at 20-42 degrees C, pH 5.5-9.5 and 0-0.7% (w/v) NaCl. Strains RG17(T) and RG53(T) contained MK-8 as main menaquinone and C-15:1 omega 6c, iso-C-15:0, and Summed Feature 3 as the major fatty acids. The genomic DNA G + C content of strains RG17(T) and RG53(T) were 61.6 and 60.7%, respectively. The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values between the isolated strains and the closely related Geomonas species were lower than the cut-off value (dDDH 70% and ANI 95-96%) for prokaryotic species delineation. Both strains possessed nif genes nifHDK and nitrogenase activities. Based on the above results, the two strains represent two novel species of the genus Geomonas, for which the names Geomonas fuzhouensis sp. nov. and Geomonas agri sp. nov., are proposed. The type strains are RG17(T) (= GDMCC 1.2687(T) = KTCC 25332(T)) and RG53(T) (= GDMCC 1.2630(T) = KCTC 25331(T)), respectively.

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