Molecular characterisation and antimicrobial resistance of Streptococcus agalactiae isolates from dairy farms in China

文献类型： 外文期刊

第一作者： Ma, Xiaojiao

作者： Ma, Xiaojiao;Shao, Wei;Ma, Xiaojiao;Chen, He;Wang, Fulan;Wang, Shuai;Wu, Yating;Ma, Xianlan;Zhao, Yankun;Ma, Xiaojiao;Chen, He;Wang, Fulan;Wang, Shuai;Wu, Yating;Ma, Xianlan;Zhao, Yankun;Ma, Xiaojiao;Chen, He;Wang, Fulan;Wang, Shuai;Wu, Yating;Ma, Xianlan;Zhao, Yankun;Wei, Yong

作者机构：

关键词： Streptococcus agalactiae; serotype; molecular characterisation; antimicrobial resistance; drug resistance gene

期刊名称：JOURNAL OF VETERINARY RESEARCH （ 影响因子：1.8； 五年影响因子：2.2 ）

ISSN： 2450-7393

年卷期： 2023 年 67 卷 2 期

页码：

收录情况： SCI

摘要： Introduction: Streptococcus agalactiae (S. agalactiae) is a pathogen causing bovine mastitis that results in considerable economic losses in the livestock sector. To understand the distribution and drug resistance characteristics of S. agalactiae from dairy cow mastitis cases in China, multilocus sequence typing (MLST) was carried out and the serotypes and drug resistance characteristics of the bacteria in the region were analysed. Material and Methods: A total of 21 strains of bovine S. agalactiae were characterised based on MLST, molecular serotyping, antimicrobial susceptibility testing, and the presence of drug resistance genes. Results: The serotypes were mainly Ia and II, accounting for 47.6% and 42.9% of all serotypes, respectively. Five sequence types (STs) were identified through MLST. The ST103 and ST1878 strains were predominant, with rates of 52.4% and 28.6%, respectively. The latter is a novel, previously uncharacterised sequence type. More than 90% of S. agalactiae strains were susceptible to penicillin, oxacillin, cephalothin, ceftiofur, gentamicin, florfenicol and sulfamethoxazole. The bacteria showed high resistance to tetracycline (85.7%), clindamycin (52.1%) and erythromycin (47.6%). Resistant genes were detected by PCR, the result of which showed that 47.6%, 33.3% and 38.1% of isolates carried the tet(M), tet(O) and erm(B) genes, respectively. Conclusion: The results of this study indicate that S. agalactiae show a high level of antimicrobial resistance. It is necessary to monitor the pathogens of mastitis to prevent the transmission of these bacteria.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序