Antibacterial characterization of Bacillus velezensis LG37 and mining of genes related to biosynthesis of antibacterial substances

文献类型： 外文期刊

第一作者： Liu, Guangxin

作者： Liu, Guangxin;Deng, Yiqin;Cheng, Changhong;Ma, Hongling;Jiang, Jianjun;Feng, Juan;Guo, Zhixun;Liu, Guangxin;Deng, Yiqin;Cheng, Changhong;Ma, Hongling;Jiang, Jianjun;Feng, Juan;Guo, Zhixun

作者机构：

关键词： Bacillus velezensis; bacteriocin; gene cluster; non-ribosomal peptide; ribosomal peptide

期刊名称：ISRAELI JOURNAL OF AQUACULTURE-BAMIDGEH （ 影响因子：0.6； 五年影响因子：0.6 ）

ISSN： 0792-156X

年卷期： 2023 年 74 卷

页码：

收录情况： SCI

摘要： Bacillus velezensis LG37 secretes various antibacterial substances and inhibits the growth of other bacteria. Here, we analyzed the antibacterial characteristics and the screening and verification of genes related to the synthesis of the antibacterial substance of LG37 by antibacterial activities experiment, Local BLAST+, and RT-PCR. LG37 was isolated from aquaculture water and preserved in our laboratory. The phylogenetic tree was used to analyze the genetic relationship between LG37 and the bacteriostatic test indicator strain. LG37 had a more substantial inhibitory effect on closely related strains, while the inhibitory effect on the more distantly related strains was weak. Combined with genome sequencing results, the ribosomal peptide (RP) bacteriocin gene and non-ribosomal peptide synthetase (NRPSs) related gene clusters were screened and analyzed. A total of six gene -coding RP bacteriocins and two genes coding surfactins and fengycin A NRPSs gene cluster were screened. Local BLAST+ analysis revealed a total of 11 NRPSs gene clusters. RT-PCR further validated the active expression of the NRPSs and RP encoding genes. The findings revealed various genes and gene clusters encoding RP bacteriocins and NRPSs in B. velezensis LG37. The bacterium is potentially valuable in diverse applications in aquaculture.

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