Functional analysis of the mating type genes in Verticillium dahliae

文献类型： 外文期刊

第一作者： Zhang, Ya-Duo

作者： Zhang, Ya-Duo;Ji, Xiao-Bin;Zong, Juan;Dai, Xiao-Feng;Zhang, Dan-Dan;Chen, Jie-Yin;Klosterman, Steven J.;Subbarao, Krishna V.;Zhang, Dan-Dan;Chen, Jie-Yin

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关键词： Verticillium dahliae; Mating type; Pheromone; Sexual reproduction; Asexual reproduction; Virulence

期刊名称：BMC BIOLOGY （ 影响因子：5.4； 五年影响因子：7.1 ）

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年卷期： 2024 年 22 卷 1 期

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收录情况： SCI

摘要： Background Populations of the plant pathogenic fungus Verticillium dahliae display a complex and rich genetic diversity, yet the existence of sexual reproduction in the fungus remains contested. As pivotal genes, MAT genes play a crucial role in regulating cell differentiation, morphological development, and mating of compatible cells. However, the functions of the two mating type genes in V. dahliae, VdMAT1-1-1, and VdMAT1-2-1, remain poorly understood. Results In this study, we confirmed that the MAT loci in V. dahliae are highly conserved, including both VdMAT1-1-1 and VdMAT1-2-1 which share high collinearity. The conserved core transcription factor encoded by the two MAT loci may facilitate the regulation of pheromone precursor and pheromone receptor genes by directly binding to their promoter regions. Additionally, peptide activity assays demonstrated that the signal peptide of the pheromone VdPpg1 possessed secretory activity, while VdPpg2, lacked a predicted signal peptide. Chemotactic growth assays revealed that V. dahliae senses and grows towards the pheromones FO-a and FO-alpha of Fusarium oxysporum, as well as towards VdPpg2 of V. dahliae, but not in response to VdPpg1. The findings herein also revealed that VdMAT1-1-1 and VdMAT1-2-1 regulate vegetative growth, carbon source utilization, and resistance to stressors in V. dahliae, while negatively regulating virulence. Conclusions These findings underscore the potential roles of VdMAT1-1-1 and VdMAT1-2-1 in sexual reproduction and confirm their involvement in various asexual processes of V. dahliae, offering novel insights into the functions of mating type genes in this species.

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