The C2H2-type zinc-finger regulator AoKap5 is required for the growth and kojic acid synthesis in Aspergillus oryzae

文献类型： 外文期刊

第一作者： Li, Yuzhen

作者： Li, Yuzhen;Chen, Ziming;Chen, Tianming;Fan, Junxia;Deng, Xin;Lei, Xiaocui;Zeng, Bin;Zhang, Zhe;Zhang, Feng;Zeng, Bin

作者机构：

关键词： Kojic acid; Zinc finger protein; Aspergillus oryzae; Secondary metabolism

期刊名称：FUNGAL GENETICS AND BIOLOGY （ 影响因子：3.0； 五年影响因子：3.3 ）

ISSN： 1087-1845

年卷期： 2023 年 167 卷

页码：

收录情况： SCI

摘要： Aspergillus oryzae is an important filamentous fungus widely used for the industrial production of fermented foods and secondary metabolites. The clarifying of the mechanism of the growth and secondary metabolites in A. oryzae is important for its industrial production and utilization. Here, the C2H2-type zinc-finger protein AoKap5 was characterized to be involved in the growth and kojic acid production in A. oryzae. The Aokap5- disrupted mutants were constructed by the CRISPR/Cas9 system, which displayed increased colony growth but decreased conidial formation. Deletion of Aokap5 enhanced the tolerance to cell-wall and oxidative but not osmotic stress. The transcriptional activation assay revealed that AoKap5 itself didn't have transcriptional activation activity. Disruption of Aokap5 resulted in the reduced production of kojic acid, coupled with the reduced expression of the kojic acid synthesis genes kojA and kojT. Meanwhile, overexpression of kojT could rescue the decreased production of kojic acid in Aokap5-deletion strain, indicating that Aokap5 serves upstream of kojT. Furthermore, the yeast one-hybrid assay demonstrated that AoKap5 could directly bind to the kojT promoter. These findings suggest that AoKap5 regulates kojic acid production through binding to the kojT promoter. This study provides an insight into the role of zinc finger protein in the growth and kojic acid biosynthesis of A. oryzae.

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