Development of a Novel Double Antibody Sandwich ELISA for Quantitative Detection of Porcine Deltacoronavirus Antigen

文献类型： 外文期刊

第一作者： Wang, Wei

作者： Wang, Wei;Li, Jizong;Fan, Baochao;Zhang, Xuehan;Guo, Rongli;Zhao, Yongxiang;Zhou, Junming;Zhou, Jinzhu;Li, Bin;Wang, Wei;Li, Jizong;Fan, Baochao;Zhang, Xuehan;Guo, Rongli;Zhao, Yongxiang;Zhou, Junming;Zhou, Jinzhu;Li, Bin;Wang, Wei;Li, Jizong;Fan, Baochao;Zhang, Xuehan;Guo, Rongli;Zhao, Yongxiang;Zhou, Junming;Zhou, Jinzhu;Li, Bin;Wang, Wei;Li, Jizong;Fan, Baochao;Zhang, Xuehan;Guo, Rongli;Zhao, Yongxiang;Zhou, Junming;Zhou, Jinzhu;Li, Bin;Sun, Dongbo

作者机构：

关键词： porcine deltacoronavirus; quantitative ELISA; antigen detection; intestinal and fecal samples; vaccine evaluation

期刊名称：VIRUSES-BASEL （ 影响因子：5.818； 五年影响因子：5.811 ）

ISSN：

年卷期： 2021 年 13 卷 12 期

页码：

收录情况： SCI

摘要： Porcine deltacoronavirus (PDCoV) can cause diarrhea and dehydration in newborn piglets. Here, we developed a double antibody sandwich quantitative enzyme-linked immunosorbent assay (DAS-ELISA) for detection of PDCoV by using a specific monoclonal antibody against the PDCoV N protein and an anti-PDCoV rabbit polyclonal antibody. Using DAS-ELISA, the detection limit of recombinant PDCoV N protein and virus titer were approximately 0.5 ng/mL and 10(3.0) TCID50/mL, respectively. A total of 59 intestinal and 205 fecal samples were screened for the presence of PDCoV by using DAS-ELISA and reverse transcriptase real-time PCR (RT-qPCR). The coincidence rate of the DAS-ELISA and RT-qPCR was 89.8%. DAS-ELISA had a sensitivity of 80.8% and specificity of 95.6%. More importantly, the DAS-ELISA could detect the antigen of PDCoV inactivated virus, and the viral antigen concentrations remained unchanged in the inactivated virus. These results suggest that DAS-ELISA could be used for antigen detection of clinical samples and inactivated vaccines. It is a novel method for detecting PDCoV infections and evaluating the PDCoV vaccine.

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