Transcriptomic analysis of Stropharia rugosoannulata reveals carbohydrate metabolism and cold resistance mechanisms under low-temperature stress

文献类型： 外文期刊

第一作者： Hao, Haibo

作者： Hao, Haibo;Wu, Shengdong;Kuai, Benke;Hao, Haibo;Wu, Shengdong;Kuai, Benke;Zhang, Jinjing;Bai, Jing;Zhuo, Xinyi;Zhang, Jiaxin;Chen, Hui

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关键词： Stropharia rugosoannulata; Transcriptomic; Carbohydrate enzymes; Antioxidant enzyme; Heat shock protein; Low-temperature stress

期刊名称：AMB EXPRESS （ 影响因子：4.126； 五年影响因子：4.245 ）

ISSN： 2191-0855

年卷期： 2022 年 12 卷 1 期

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收录情况： SCI

摘要： Low temperature is an important environmental factor that restricts the growth of Stropharia rugosoannulata; however, the molecular mechanisms underlying S. rugosoannulata responses to low-temperature stress are largely unknown. In this study, we performed a transcriptome analysis of a high-sensitivity strain (DQ-1) and low-sensitivity strain (DQ-3) under low-temperature stress. The liquid hyphae of S. rugosoannulata treated at 25 degrees C and 10 degrees C were analyzed by RNA-Seq, and a total of 9499 differentially expressed genes (DEGs) were identified. GO and KEGG enrichment analyses showed that these genes were enriched in "xenobiotic biodegradation and metabolism", "carbohydrate metabolism", "lipid metabolism" and "oxidoreductase activity". Further research found that carbohydrate enzyme (AA, GH, CE, and GT) genes were downregulated more significantly in DQ-1 than DQ-3 and several cellulase activities were also reduced to a greater extent. Moreover, the CAT1, CAT2, GR, and POD genes and more heat shock protein genes (HSP20, HSP78 and sHSP) were upregulated in the two strains after low-temperature stress, and the GPX gene and more heat shock protein genes were upregulated in DQ-3. In addition, the enzyme activity and qRT-PCR results showed trends similar to those of the RNA-Seq results. This result indicates that low-temperature stress reduces the expression of different AA, GH, CE, and GT enzyme genes and reduces the secretion of cellulase, thereby reducing the carbohydrate metabolism process and mycelial growth of S. rugosoannulata. Moreover, the expression levels of different types of antioxidant enzymes and heat shock proteins are also crucial for S. rugosoannulata to resist low-temperature stress. In short, this study will provide a basis for further research on important signaling pathways, gene functions and variety breeding of S. rugosoannulata related to low-temperature stress.

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