High-throughput screening, "protein-metabolite" interaction, and hypoglycemic effect investigations of α-amylase inhibitors in teas using an affinity selection-mass spectrometry method

文献类型： 外文期刊

第一作者： Wang, Zhe

作者： Wang, Zhe;Gao, Jianjian;Zhou, Mengxue;Chen, Dan;Zhou, Chuang;Yu, Shuai;Lin, Zhiyuan;Peng, Jiakun;Lin, Zhi;Dai, Weidong;Wang, Zhe;Zhao, Yanni;Chen, Dan;Zhou, Chuang

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关键词： Affinity selection-mass spectrometry; Theaflavins; Amylase inhibitor; Hypoglycemic effect; Tea

期刊名称：LWT-FOOD SCIENCE AND TECHNOLOGY （ 影响因子：6.0； 五年影响因子：6.0 ）

ISSN： 0023-6438

年卷期： 2024 年 203 卷

页码：

收录情况： SCI

摘要： Tea is consumed worldwide and can reduce the risk of diabetes whereas the hypoglycemic compounds in tea remain unclear. Herein, the chemical compositions comparison and inhibition assays indicated that the inhibitory effect of tea against alpha-amylase was positively related with the enzyme-mediated oxidation degree of tea and black teas containing high contents of theaflavins exhibited the strongest in vitro inhibitory effect. Affinity selection-mass spectrometry-based high-throughput screening revealed that four theaflavins specifically bind to alpha-amylase. Compared to other compounds, theaflavins showed one order of magnitude higher in vitro inhibitory effects; thus, theaflavins are possibly the dominant alpha-amylase inhibitors in tea. A series of "alpha-amylase-theaflavins" interaction studies jointly demonstrated that theaflavins noncompetitively inhibit alpha-amylase activity by interacting with the amino acid residues of alpha-amylase and then inducing changes in the secondary structure. Furthermore, theaflavins (10 mg/kg/d) and black tea extracts (100 mg/kg/d) exhibited comparable hypoglycemic effects as acarbose in diabetic mice.

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