Simultaneous quantification of two commonly used anticoagulant drugs and three hypolipidemic drugs in human plasma using gradient high-performance liquid chromatography

文献类型： 外文期刊

第一作者： Liu, Fuyan

作者： Liu, Fuyan;Hao, Zhaoyang;Lei, Mingxing;Zhang, Xiao-Feng;Zhang, Haolin;Li, Xia;Shao, Huarong;Ling, Peixue;Zhang, Xiao-Feng

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关键词： Simultaneous quantification; anticoagulant drugs; hypolipidemic drugs; gradient high-performance liquid chromatography

期刊名称：JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES （ 影响因子：1.0； 五年影响因子：1.4 ）

ISSN： 1082-6076

年卷期： 2024 年

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收录情况： SCI

摘要： The combination of an anticoagulant and a hypolipidemic agent is commonly used in patients at risk for stroke because it controls thrombosis and lowers cholesterol levels. However, the use of anticoagulants carries a high risk of life-threatening bleeding. To address the need for personalized clinical monitoring and risk management, we developed a rapid and sensitive HPLC method with a streamlined sample preparation process. This method enables the simultaneous determination of concentrations for two commonly used anticoagulant drugs (apixaban and rivaroxaban) and three hypolipidemic drugs (rosuvastatin, atorvastatin calcium, and simvastatin). The chromatographic conditions and sample preparation methods were optimized. The drugs were well separated by the gradient elution of phosphoric acid (0.01%, v/v) and acetonitrile at a flow rate of 1.0 mL/min within 40 min on an Athena C18-WP column (4.6 x 150 mm, 5-mu m particle size). The proposed method was linear (r(2) >= 0.994) within the concentration ranges of 0.22-20 mu gmL(-1) for the five analytes, including the expected range of therapeutic concentrations. This method enables simultaneous monitoring of the levels of the mentioned drugs in clinical practice. Furthermore, the chromatographic conditions were simple and feasible, making them highly suitable for emergency clinical medication monitoring in basic medical institutions.

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