Isolation, pathogenesis, and genetic evolution of a porcine bocavirus PBoV/ HB/30/2018 strain in China

文献类型： 外文期刊

第一作者： Aryal, Manita

作者： Aryal, Manita;Huang, Xin;Fu, Yuguang;Li, Yang;Wang, Bin;Chen, Qingbo;Yan, Maiomaio;Chen, Jianing;Liu, Guangliang;Liu, Guangliang

作者机构：

关键词： Porcine bocavirus; Pathogenesis; Diarrhea; Phylogeny evaluation

期刊名称：VIROLOGY （ 影响因子：3.513； 五年影响因子：3.479 ）

ISSN： 0042-6822

年卷期： 2022 年 572 卷

页码：

收录情况： SCI

摘要： Porcine bocavirus (PBoV) was first identified in Sweden in 2009. Due to its association with healthy as well as diseased pigs, its role in clinical disease has not been reported yet. In the present study, bocavirus was identified from the intestinal content of a 30-day-old piglet and its whole genome was constructed and phylogenetic analysis was carried on. The pathogenesis of bocavirus was investigated following orogastric inoculation of the colostrum-deprived newborn piglet with bacteria free intestinal content. The bocavirus-inoculated piglets developed diarrhea, shed virus in the rectal swabs from 18 h post inoculation and developed macroscopic and microscopic lesions in small intestine with virus confirmed by conventional PCR. This study experimentally confirmed pathogenicity and characterized bocavirus as the etiological agent of diarrhea in the colostrum deprived newborn piglets. On phylogenetic analysis, it was observed that this virus has long evolutionary history with subsequent mutation as well as better host adaptation. This study highlights the importance of identifying bocavirus as the etiological agent of viral diarrhea that could threaten livestock, public health as well as economic loss.

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