Enhancing butyrate synthesis and intestinal epithelial energy supply through mixed probiotic intervention in dextran sulfate sodium-induced colitis

文献类型： 外文期刊

第一作者： Zhang, Heng

作者： Zhang, Heng;Sun, Jingzhou;Xie, Aowen;Yang, Huiqin;Li, Yingjun;Mei, Yuxia;Li, Jinshan;Xiao, Lei;Liu, Yangyang;Liang, Yunxiang;Zhang, Heng;Sun, Jingzhou;Xie, Aowen;Yang, Huiqin;Li, Yingjun;Mei, Yuxia;Li, Jinshan;Xiao, Lei;Liu, Yangyang;Liang, Yunxiang;Liu, Yangyang

作者机构：

关键词： Probiotics; Gut microbiota; Butyrate synthesis; Inflammatory bowel disease; Energy supply

期刊名称：FOOD BIOSCIENCE （ 影响因子：5.9； 五年影响因子：6.1 ）

ISSN： 2212-4292

年卷期： 2025 年 63 卷

页码：

收录情况： SCI

摘要： Inflammatory bowel disease (IBD) is a globally prevalent gastrointestinal disorder that considerably impairs life quality. Probiotics are considered potential therapeutic agents for IBD because of their multifaceted effects. In this study, we evaluated the therapeutic effects of a mixture of probiotics (Lactobacillus rhamnosus, Lactobacillus plantarum, and Clostridium butyricum) on dextran sulfate sodium (DSS)-induced colitis in mice. To determine its anti-inflammatory mechanism, we employed metagenomic sequencing to analyze the effects of mixed probiotics on butyrate-producing bacteria and related genes in the gut. Immunofluorescence, immunohistochemistry, and flow cytometry were used to detect the effects of mixed probiotics on the intestinal barrier, immune function, and mitochondrial function in mice. Finally, in vitro fermentation experiments were conducted to evaluate the antiinflammatory effects of the intestinal flora in mice relative to antibiotics. Our findings demonstrated that mixed probiotics enhance the conversion of lactate to butyrate by restoring the acetyl-CoA pathway in the colonic microbiota of mice with DSS-induced colitis. This restoration, in turn, led to the restoration of butyrate levels in the intestine and the activation of the butyrate receptor peroxisome proliferator-activated receptor gamma (PPAR-gamma) to sustain the energy supply of intestinal epithelial cells. Consequently, this intervention reinforced the intestinal barrier integrity and modulated intestinal immune responses. In conclusion, mixed probiotics alleviate colitis, providing a potential therapeutic strategy for IBD.

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