Efficient generation of targeted point mutations in the Brassica oleracea var. botrytis genome via a modified CRISPR/Cas9 system

文献类型： 外文期刊

第一作者： Wang, Guixiang

作者： Wang, Guixiang;Zong, Mei;Liu, Di;Wu, Yage;Tian, Shouwei;Han, Shuo;Guo, Ning;Duan, Mengmeng;Miao, Liming;Liu, Fan

作者机构：

关键词： Cauliflower; Targeted point mutations; Base-editing; CRISPR; Cas9; ALS; CENH3

期刊名称：HORTICULTURAL PLANT JOURNAL （ 影响因子：4.24； ）

ISSN： 2095-9885

年卷期： 2022 年 8 卷 4 期

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收录情况： SCI

摘要： A B S T R A C T In this study, we used the modified CRISPR/Cas9 system to produce targeted point mutations in cauliflower. Acetolactate synthase ( ALS ) and Centromere-specific histone H3 variant (CENH3) genes were selected as the base-editing targets and hypocotyls of cauliflower were used as explants. For ALS gene, a C-to-T conversion in the Pro182 codon (CCT) can alter the encoded amino acid, likely resulting in herbicide resistance, and a C-to-T mutation in the Leu133 codon (CTT) in the CENH3 gene may produce a haploid inducer. Results indicated that the transformation efficiency was 1.8%-4.5% and the mutation efficiencies for the ALS and CENH3 genes were approximately 22% and 87%, respectively. The ALS mutant cauliflower showed strong herbicide resistance, with possible immediate implications for broadleaf weed control in cauliflower fields.

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