Recombinant Pichia pastoris overexpressing bioactive phytase

文献类型： 外文期刊

第一作者： Yao, B

作者： Yao, B;Zhang, CY;Wang, JH;Fan, YL

作者机构：

关键词： phytase gene phyA2;recombinant Pichia pastoris;overexpression

期刊名称：SCIENCE IN CHINA SERIES C-LIFE SCIENCES （ 影响因子：1.61； 五年影响因子：1.148 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Phytase gene pfiyA2, whose signal peptide encoding sequence and intion sequence had been removed, was modified. The Arg-encoding codons CGG and CAG in phyA2 were mutated into synonymous codon AGA The modified phyA2 was fused behind alpha-factor signalsequence under the control of AOX1 promoter in plasmid pPIC9, then introduced into the host Pichia pastoris by electroporation. The results of Southern blotting analysis and Northern blotting analysis demonstrated that the phyA2 gene had integrated intothe genome of P. pastoris and transcribed. The result of SDS-PAGE of the phytase expressed by P. pastoris showed that the modified phyA2 had been overexpressed and secreted. The concentration of the phytase expressed by P. pastoris with modified phyA2 exceeded 15 000 U/mL, which had a 3 000-fold increase over that of origin Aspergillus niger 963 and was 37 times higher than that of recombinant P. pastoris with non-modified phyA2.

分类号： Q1

  • 相关文献

[1]Overexpression of cotton (Gossypium hirsutum) dirigent1 gene enhances lignification that blocks the spread of Verticillium dahliae. Haiyan Shi,Zhihao Liu,Li Zhu,Chaojun Zhang,Yun Chen,Ying Zhou,Fuguang Li,Xuebao Li. 2012

[2]Molecular Cloning and Function Analysis of Two SQUAMOSA-Like MADS-Box Genes From Gossypium hirsutum L.. Wenxiang Zhang,Shuli Fan,Chaoyou Pang,Hengling Wei,Jianhui Ma,Meizhen Song,Shuxun Yu. 2013

[3]Virus-Based MicroRNA Silencing and Overexpressing in Common Wheat (Triticum aestivum L.). Jian, Chao,Chi, Qing,Wang, Shijuan,Ma, Meng,Liu, Xiangli,Zhao, Huixian,Han, Ran,Zhao, Huixian. 2017

[4]Isolation and characterization of a stress responsive small GTP-binding protein AhRabG3b in peanut (Arachis hypogaea L.). Yu, Shan-Lin,Sui, Jiong-Ming,Li, Rui,Fan, Qian-Cheng,Zheng, Chun-Hua,Wang, Jing-Shan,Qiao, Li-Xian,Song, Lin. 2013

[5]Expression regulation of a xylanase inhibitor gene riceXIP in rice (Oryza sativa L.). Zhan, Yihua,Sun, Xiangyu,Xu, Ying,Huang, Yingying,Jiang, Dean,Weng, Xiaoyan,Sun, Renjie,Hou, Chunxiao,Hou, Chunxiao. 2017

[6]Effect of the expression and knockdown of citrate synthase gene on carbon flux during triacylglycerol biosynthesis by green algae Chlamydomonas reinhardtii. Cai, Jiajia,Fei, Xiaowen,Deng, Xiaodong. 2013

[7]Characterization of a pathway-specific activator of milbemycin biosynthesis and improved milbemycin production by its overexpression in Streptomyces bingchenggensis. He, Hairong,Liu, Hui,Wang, Haiyan,Xiang, Wensheng,He, Hairong,Liu, Hui,Wang, Xiangjing,Xiang, Wensheng. 2016

[8]ATP-Citrate Lyase Gene (SoACLA-1), a Novel ACLA Gene in Sugarcane, and Its Overexpression Enhance Drought Tolerance of Transgenic Tobacco. Li, Jian,Sun, Bo,Liu, Jia-Yi,Zhao, Wen-Hui,Huang, Chan,Yang, Li-Tao,Li, Yang-Rui,Yang, Li-Tao,Li, Yang-Rui. 2017

[9]Cloning, Expression, and Characterization of a New Streptomyces sp S27 Xylanase for Which Xylobiose is the Main Hydrolysis Product. Li, Ning,Shi, Pengjun,Yang, Peilong,Wang, Yaru,Luo, Huiying,Bai, Yingguo,Zhou, Zhigang,Yao, Bin. 2009

[10]Expression and functional characterization of a gene associated with retinoid-interferon-induced mortality 19 (GRIM-19) from orange-spotted grouper (Epinephelus coioides). Shi, Yan,Zhu, Xinping,Chen, Kunci,Zhao, Zhe,Zhang, Qiya. 2013

[11]A wheat PI4K gene whose product possesses threonine autophophorylation activity confers tolerance to drought and salt in Arabidopsis. Liu, Pei,Xu, Zhao-Shi,Lu, Pan-Pan,Hu, Di,Chen, Ming,Li, Lian-Cheng,Ma, You-Zhi. 2013

[12]Gene Cloning, Overexpression, and Characterization of a Xylanase from Penicillium sp CGMCC 1669. Liu, Wanli,Shi, Pengjun,Yang, Peilong,Wang, Guozeng,Wang, Yaru,Luo, Huiying,Yao, Bin,Liu, Wanli,Chen, Qiang. 2010

[13]Transcript characteristic of myostatin in sheep fibroblasts. Lu, Jian,Ding, Jiatong,Li, Bichun,Lu, Jian,Zhang, Xiaoning,Zhao, Fuping,Zhou, Xinlei,Zhang, Li,Wei, Caihong,Du, Lixin,Ren, Hangxing,Sheng, Xihui,Li, Shangang.

[14]Overexpression of a Lotus corniculatus AP2/ERF transcription factor gene, LcERF080, enhances tolerance to salt stress in transgenic Arabidopsis. Sun, Zhan-Min,Zhou, Mei-Liang,Tang, Yi-Xiong,Wu, Yan-Min,Sun, Zhan-Min,Xiao, Xing-Guo.

[15]The role of thionins in rice defence against root pathogens. Gheysen, Godelieve,Ullah, Chhana,Verbeek, Ruben,Shang, Chenjing,Kyndt, Tina,Ji, Hongli,Verbeek, Ruben,De Vleesschauwer, David,Hofte, Monica.

[16]Molecular cloning, characterization, and function analysis of a mevalonate pyrophosphate decarboxylase gene from Ganoderma lucidum. Shi, Liang,Qin, Lei,Ren, Ang,Fang, Xing,Mu, Dashuai,Zhao, Mingwen,Xu, Yingjie,Tan, Qi.

[17]Increased salt tolerance with overexpression of cation/proton antiporter 1 genes: a meta-analysis. Ma, Yuan-Chun,Ma, Yuan-Chun,Dong, Chao,Cheng, Zong-Ming (Max),Ma, Yuan-Chun,Auge, Robert M.,Cheng, Zong-Ming (Max). 2017

[18]High-level expression of a novel Penicillium endo-1,3(4)-beta-D-glucanase with high specific activity in Pichia pastoris. Chen, Xiaoyan,Meng, Kun,Shi, Pengjun,Bai, Yingguo,Luo, Huiying,Huang, Huoqing,Yuan, Tiezheng,Yang, Peilong,Yao, Bin.

[19]Overexpression of the novel Zygophyllum xanthoxylum C2H2-type zinc finger gene ZxZF improves drought tolerance in transgenic Arabidopsis and poplar. Chu, Yanguang,Zhang, Weixi,Huang, Qinjun,Zhang, Bingyu,Su, Xiaohua,Chu, Yanguang,Zhang, Weixi,Huang, Qinjun,Zhang, Bingyu,Su, Xiaohua,Wu, Bin.

[20]Optimized gene synthesis, expression and purification of active salivary plasminogen activator alpha 2 (DSPAot2) of Desmodus rotundus in Pichia pastoris. Wei, Zhaorong,Li, Gangqiang,Liu, Dehu,Wang, Yueju,Li, Xiaokun,Liu, Dehu.

作者其他论文 更多>>

微信小程序