Histone variant H2AZ1 drives lung cancer progression through the RELA-HIF1A-EGFR signaling pathway

文献类型： 外文期刊

第一作者： Zhao, Huijie

作者： Zhao, Huijie;Wu, Xing;Wang, Yinghan;Du, Yuhui;Zhou, Zhiqing;Li, Yu;Liu, Yue;Zeng, Xiaofei;Chen, Guoan;Zhao, Huijie;Li, Xiuling;Zeng, Xiaofei;Chen, Guoan

作者机构：

关键词： Lung cancer; H2AZ1; RELA; HIF1A; EGFR

期刊名称：CELL COMMUNICATION AND SIGNALING （ 影响因子：8.9； 五年影响因子：9.1 ）

ISSN：

年卷期： 2024 年 22 卷 1 期

页码：

收录情况： SCI

摘要： BackgroundA growing body of evidence indicates that histone variants play an oncogenic role in cancer progression. However, the role and mechanism of histone variant H2AZ1 in lung cancer remain poorly understood. In this study, we aim to identify novel functions and molecular mechanisms of H2AZ1 in lung cancer.MethodsWe analyzed H2AZ1 expression in lung adenocarcinoma using several RNA-seq and microarray datasets. Immunohistochemistry staining for H2AZ1 was performed on two sets of lung cancer tissue microarrays. To study the function of H2AZ1, we conducted assays for cell proliferation, colony formation, invasion, and migration. We employed CUT&Tag-seq, ATAC-seq, RNA-seq, and Western blotting to explore the regulatory patterns and potential mechanisms of H2AZ1 in lung adenocarcinoma.ResultsOur findings reveal that H2AZ1 is highly expressed in lung cancer and high levels of H2AZ1 mRNA are associated with poor patient survival. Silencing H2AZ1 impaired cell proliferation, colony formation, migration, and invasion. Mechanistically, our CUT&Tag-seq, ATAC-seq, and RNA-seq results showed that H2AZ1 is primarily deposited around TSS and affects multiple oncogenic signaling pathways. Importantly, we uncovered that H2AZ1 may drive lung cancer progression through the RELA-HIF1A-EGFR signaling pathway.ConclusionH2AZ1 plays an oncogenic role via several cancer-related pathways, including the RELA-HIF1A-EGFR axis in lung cancer. Intervention targeting H2AZ1 and its related signaling genes may have translational potential for precision therapy.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序