Functional Characterization of Apple MdTGA2.1 in Arabidopsis with Reduced SA Sensitivity and Increased Stress Tolerance

文献类型： 外文期刊

第一作者： Tian, Yi

作者： Tian, Yi;Zhang, Cai-Xia;An, Xiu-Hong;Cong, Pei-Hua;Tian, Yi;Zhu, Chen-Yu;An, Xiu-Hong

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关键词： Apple; TGA transcription factor; Protein interaction; Transgenic Arabidopsis; SA response; Stress response

期刊名称：JOURNAL OF PLANT GROWTH REGULATION （ 影响因子：4.64； 五年影响因子：4.469 ）

ISSN： 0721-7595

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收录情况： SCI

摘要： Salicylic acid (SA) is a plant defense hormone required for immunity. Group II TGA transcription factors could interact with NPR1 to positively regulate SA-induced PR gene expression and pathogen resistance. In this study, one group II TGA gene from apple, MdTGA2.1, was functionally characterized detailed. The expression of MdTGA2.1 was mainly expressed in the leaves and up-regulated by SA treatment. MdTGA2.1-GFP fusion protein was observed only in the nucleus in the transient expression assay. Yeast two-hybrid and pull-down assays revealed that MdTGA2.1 could interact with MdNPR1. Overexpression of MdTGA2.1 in the tga6-1tga2-1tga5-1 triple mutant and wild-type Col conferred decreased SA sensitivity, including the SA-mediated seedlings growth inhibition, and the susceptibility to the bacterial pathogen Pst DC3000. In addition, MdTGA2.1 overexpression also improved the tolerance to NaCl and PEG treatments in transgenic Arabidopsis. Taken together, our findings suggest that apple MdTGA2.1 not only involved in the SA responses but also played roles in the stress tolerance.

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