Arabidopsis thaliana E3 ligase AIRP4 is involved in GA synthesis

文献类型： 外文期刊

第一作者： Wang, Tao

作者： Wang, Tao;Zhou, Qin;Wu, Xiaobo;Wang, Duo;Luo, Wenmin;Wang, Jianmei;Yang, Yi;Liu, Zhibin;Yang, Liang

作者机构：

关键词： Endogenous GA 3; AtAIRP4; Hypocotyl extension; PAC

期刊名称：JOURNAL OF PLANT PHYSIOLOGY （ 影响因子：3.686； 五年影响因子：4.352 ）

ISSN： 0176-1617

年卷期： 2022 年 277 卷

页码：

收录情况： SCI

摘要： Arabidopsis abscisic acid ABA-Insensitive RING Proteins (AtAIRP1-4) are RING E3s that play significant roles in ABA-signaling pathways. However, it is still unclear whether they have other functions. Here, AtAIRP4 was determined to play a role in response to gibberellin A3 (GA3) in Arabidopsis thaliana. After proAtAIRP4::GUS transgenic lines were treated with GA3, the GUS activity decreased in hypocotyls. Increased hypocotyl elongation in response to GA3 seen in WT was not observed in the AtAIRP4-overexpression lines, whereas AtAIRP4-overexpression lines were hypersensitive to Paclobutrazol (PAC, an inhibitor of GA biosynthesis) during the seed germination stage. Additionally, AtAIRP4-overexpressing lines showed the lowest level of primary root elongation in the presence of GA3. The levels of endogenous GA3 in 35S::AtAIRP4 lines were lower than those in wildtype. In addition, among the plants, the mRNA levels of the GA synthetic gene GIBBERELLIN 20-OXIDASE1 (GA20ox1) was the lowest in overexpressing line. However, the expression of the response gene DELLA RGALIKE3 (RGL3) was the highest in overexpressing lines after treatment with GA3. Thus, AtAIRP4 plays a negative role in GA-mediated hypocotyl elongation and root growth, and it inhibits the synthesis of endogenous biologically active GA3 to some extent.

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