Constitutive expression of cucumber CsACS2 in Arabidopsis Thaliana disrupts anther dehiscence through ethylene signaling and DNA methylation pathways

文献类型： 外文期刊

第一作者： Yang, Zonghui

作者： Yang, Zonghui;Li, Libin;Meng, Zhaojuan;Zhu, Lixia;Cao, Qiwei;Wang, Mingqi;Gao, Tian;Li, Jingjuan

作者机构：

关键词： Cucumis sativus; Arabidopsis thaliana; ACC synthase; Ethylene; Anther dehiscence; DNA methylation

期刊名称：PLANT CELL REPORTS （ 影响因子：4.5； 五年影响因子：6.1 ）

ISSN： 0721-7714

年卷期： 2024 年 43 卷 12 期

页码：

收录情况： SCI

摘要： Key messageConstitutive expression of cucumber CsACS2 in Arabidopsis disrupts anther dehiscence and male fertility via ethylene signaling and DNA methylation, revealing new avenues for enhancing crop reproductive traits.AbstractThe cucumber gene CsACS2, encoding ACC (1-aminocyclopropane-1-carboxylic acid) synthase, plays a pivotal role in ethylene biosynthesis and sex determination. This study investigates the effects of constitutive CsACS2 expression in Arabidopsis thaliana on anther development and male fertility. Transgenic Arabidopsis plants overexpressing CsACS2 exhibited male sterility due to inhibited anther dehiscence, which was linked to suppressed secondary cell wall thickening. RNA-Seq analysis revealed upregulation of ethylene signaling pathway genes and downregulation of secondary cell wall biosynthesis genes, with gene set enrichment analysis indicating the involvement of DNA methylation. Rescue experiments demonstrated that silver nitrate (AgNO3) effectively restored fertility, while 5-azacytidine (5-az) partially restored it, highlighting the roles of ethylene signaling and DNA methylation in this process. Constitutive CsACS2 expression in Arabidopsis disrupts anther development through ethylene signaling and DNA methylation pathways, providing new insights into the role of ethylene in plant reproductive development and potential applications in crop improvement.

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