Emergence of ciprofloxacin-and tigecycline-resistant extended-spectrum β-lactamase (ESBL)-producing Salmonella enterica serovar Kentucky ST198 from horse, China

文献类型： 外文期刊

第一作者： Zhang, Xing-Xing

作者： Zhang, Xing-Xing;Huang, Xin;Li, Xue-Qin;Li, Qiuchun;Zhong, Fa-Gang;Zhang, Xing-Xing;Wang, Zhen-Yu;Jiang, Yue;Jiao, Xinan;Li, Qiuchun;Wang, Jing

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关键词： CTX-M; Salmonella enterica serovar Kentucky; ST198; Extensively drug-resistant horse

期刊名称：JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE （ 影响因子：3.2； 五年影响因子：3.8 ）

ISSN： 2213-7165

年卷期： 2025 年 44 卷

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收录情况： SCI

摘要： Objectives: Salmonella enterica serovar Kentucky ST198 has been increasingly reported in diverse sources worldwide, emerging as a globally epidemic clone. In this study, we aim to characterise the first isolated S . Kentucky ST198 strain from a horse in China. Methods: Twelve faecal samples were collected from horses at a farm in Shihezi, Xinjiang Province, China, and investigated for the presence of Salmonella . The minimum inhibitory concentrations of 15 antimicrobial agents were determined using the broth microdilution method. The whole genome of Sal140 was sequenced using the Illumina Hiseq platform and assembled into contigs with SPAdes 3.10.0. Phylogenetic analysis was performed using Parsnp based on core-genome single-nucleotide polymorphisms. Results: A single S . Kentucky strain, designated Sal140, was isolated. This strain carried 15 antimicrobial resistance genes and exhibited resistance to ampicillin, ceftazidime, cefuroxime, cefotaxime, gentamicin, streptomycin, tetracycline, tigecycline, florfenicol, nalidixic acid, ciprofloxacin, azithromycin, and sulfamethoxazole/trimethoprim. Co-resistance to ciprofloxacin and third-generation cephalosporins was attributed to mutations in gyrA (S83F and D87G) and parC (T57S and S80I), and the production of CTXM-55, respectively. Phylogenetic analysis revealed that Sal140 clustered within the subclade ST198.2-2, showing close genetic similarity (15-24 SNPs) to isolates from a patient and chicken meat in other regions of China, suggesting a potential epidemiological link among these S . Kentucky ST198 isolates from different sources. Conclusions: In conclusion, the emergence of extensively drug-resistant (XDR) S . Kentucky ST198 in a horse is concerning. Continuous surveillance of this particular clone in horses is strongly recommended. (c) 2025 The Author(s). Published by Elsevier Ltd on behalf of International Society for Antimicrobial Chemotherapy. This is an open access article under the CC BY-NC-ND license ( http://creativecommons.org/licenses/by-nc-nd/4.0/ )

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