Strategy of Dimercaptothiol as Self-assembled Monolayers Enhance the Sensitivity of SPR Immunosensor for Detection of Salbutamol

文献类型： 外文期刊

第一作者： Li, Wenjie

作者： Li, Wenjie;Wang, Chenchen;Li, Huidong;Chen, Zilei;Yan, Mengmeng

作者机构：

关键词： Surface plasmon resonance; thiol; self-assembled monolayers; salbutamol

期刊名称：ANALYTICAL SCIENCES （ 影响因子：2.081； 五年影响因子：1.676 ）

ISSN： 0910-6340

年卷期： 2021 年 37 卷 9 期

页码：

收录情况： SCI

摘要： The formation of self-assembled monolayers (SAMs) is a normal method for the immobilization of biorecognition elements immobilized on SPR sensors. With this method, mercaptopropionic acid (MPA) with carboxylic and thiol group is the most commonly used. Dimercaptosuccinic acid (DMSA) having two carboxylics and two thiol groups is a classical antidote for heavy metal ions. In this paper, DMSA was first used to form SAMs to connect the antigen on the chip of a surface plasmon resonance (SPR) immunosensor for detection of salbutamol (SAL), and the results were compared with a traditional (MPA)-SPR sensor. Dihydrolipoic acid(DHLA)-SPR showed that the recognition efficiency of antigen and antibody of DMSA-SPR immunosensor was 170.1% at room temperature with the linear range of 5 - 150 ng/mL. The recovery rate of this sensor applied to SAL detection in pork reached 94.9 - 108.0% and the limit of quantification (LOQ) was 5 ng/mL. The results were in good correlation with the analysis results of ultra-high phase liquid chromatography- tandem mass spectrometry (UPLC-MS/MS) analysis. This novel DMSA-SPR immunosensor provides insight into a new idea and method for improving the sensitivity of SPR immunosensors and can be widely used in the detection of other small molecules.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序