A metabolic enzyme-photosynthetic machinery involved in the co-metabolism of enrofloxacin and ciprofloxacin by Chlorella pyrenoidosa

文献类型： 外文期刊

第一作者： Wu, Hengyu

作者： Wu, Hengyu;Xiong, Qian;Tian, Fei;Chen, Haigang;Wu, Hengyu;Wang, Yichun;Xie, Fengqi;Ma, Jiaru;Tang, Qinglin;Chen, Yanfen;Sun, Yuehong;Li, Hao;Liu, Yousheng;Ying, Guangguo;Wu, Hengyu;Wang, Yichun;Xie, Fengqi;Ma, Jiaru;Tang, Qinglin;Chen, Yanfen;Sun, Yuehong;Li, Hao;Liu, Yousheng;Ying, Guangguo

作者机构：

关键词： Fluoroquinolones; Co-metabolism; Microalgae; Transformation pathway; Key functional genes

期刊名称：JOURNAL OF HAZARDOUS MATERIALS （ 影响因子：11.3； 五年影响因子：12.4 ）

ISSN： 0304-3894

年卷期： 2025 年 492 卷

页码：

收录情况： SCI

摘要： The removal of fluoroquinolone antibiotics from wastewater continues to pose significant challenges, as conventional treatment methods often prove ineffective against these persistent pollutants. However, microalgalmediated treatment has emerged as a promising alternative, leveraging its unique potential to degrade recalcitrant contaminants. This study investigates the removal of enrofloxacin (EFX) and ciprofloxacin (CFX) by Chlorella pyrenoidosa, integrating transcriptomics, gene network analysis, and co-metabolic pathways to unravel the mechanisms driving pollutant degradation. Among the four co-metabolic substrates evaluated, glucose and glycine were identified as the most effective in enhancing the degradation of EFX and CFX, respectively. Glycine primarily upregulated genes associated with nitrogen metabolism, while glucose stimulated both photosynthesis and nitrogen metabolism pathways. This synergistic co-metabolic interaction promoted the development of an integrated metabolic enzyme-photosynthetic machinery, which enhanced electron transport, energy generation, catalytic enzyme expression, and extracellular polymeric substance (EPS) production, ultimately leading to a significant increase in the degradation rates of EFX and CFX. Mass balance analysis revealed that biotransformation processes, including defluorination, decarboxylation, hydroxylation, and other transformations, were the predominant mechanism for pollutant removal. Fluorine was detected within microalgal cells using transmission electron microscopy coupled with energy dispersive X-ray spectroscopy (TEM-EDS). A total of eight transformation products (TPs) were identified, and their non-toxic effects on three tested organisms suggest environmentally benign outcomes. These findings provide valuable insights into the mechanisms underlying microalgae-mediated degradation of fluoroquinolone antibiotics and highlight the potential of microalgaebased technologies a sustainable solution for mitigating antibiotic pollution in wastewater.

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