Mechanism on the Allergenicity Changes of alpha-Lactalbumin Treated by Sonication-Assisted Glycation during In Vitro Gastroduodenal Digestion

文献类型： 外文期刊

第一作者： Wang, Xu-Mei

作者： Wang, Xu-Mei;Tu, Zong-Cai;Wang, Hui;Hu, Yue-Ming;Tu, Zong-Cai;Ye, Yun-Hua;Tu, Zong-Cai;Ye, Yun-Hua;Liu, Guang-Xian

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关键词： alpha-lactalbumin; sonication-assisted glycation; allergenicity; high-resolution mass spectrometry; gastroduodenal digestion

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：5.279； 五年影响因子：5.269 ）

ISSN： 0021-8561

年卷期： 2021 年 69 卷 24 期

页码：

收录情况： SCI

摘要： Physical-assisted chemical modification is effective to reduce the allergenicity of alpha-lactalbumin (ALA). However, there are few in-depth studies on the allergenicity changes of physical-assisted chemical-modified ALA during digestion. The effect of gastroduodenal digestion on the allergenicity changes of ALA treated by sonication-assisted glycation was assessed. Digestion of both ALA and its glycated forms generated peptide fractions, and intact undigested glycated ALA in the hydrolysates still covalently bound to D-galactose. High-resolution mass spectrometry revealed that a higher glycation degree was discovered in sonication-preprocessed ALA compared to native ALA. Enzyme-linked immunosorbent assay and basophil degranulation showed that sonication-assisted glycation could significantly reduce ALA allergenicity. The allergenicity of both gastric and gastroduodenal hydrolysates was further increased, and the hydrolysates of sonication-assisted glycated ALA showed the lowest allergenicity. The reason could be the shielding effect of the linear epitope found to be caused by a higher glycation degree; although linear epitopes were exposed, D-galactose covalently bound to intact undigested glycated ALA in the hydrolysates retained its masking role. These results indicated that sonication-assisted glycation could be a promising method to prepare immunotherapeutic agents for allergen immunotherapy to achieve the purpose of allergy desensitization.

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