Determination of dietary copper requirement by the monoamine oxidase activity in kidney of broilers from 1 to 21 d of age

文献类型： 外文期刊

第一作者： Hu, Yun

作者： Hu, Yun;Luo, Xugang;Chen, Zhiyong;Lu, Lin;Zhang, Liyang;Liao, Xiudong;Liu, Tao

作者机构：

关键词： Copper; Monoamine oxidase; Requirement; Broiler

期刊名称：ANIMAL NUTRITION （ 影响因子：5.285； 五年影响因子：7.059 ）

ISSN： 2405-6383

年卷期： 2022 年 8 卷

页码：

收录情况： SCI

摘要： The current dietary copper (Cu) requirement (8 mg/kg) of broilers is mainly based on growth, hemoglobin concentration, or hematocrit, which might not be the most sensitive indices to evaluate dietary Cu requirements of broilers. The present study was carried out to estimate dietary Cu requirements of broilers fed a conventional corn-soybean meal diet from 1 to 21 d of age using biochemical or molecular biomarkers. A total of 384 one-day-old Arbor Acres male broilers were randomly allocated to 1 of 6 treatments with 8 replicates and fed a Cu-unsupplemented corn-soybean meal basal diet containing 5.17 mg Cu/kg by analysis and the basal diet supplemented with 3, 6, 9,12 or 15 mg Cu/kg as CuSO4 center dot 5H(2)O for 21 d. Regression analysis was performed to estimate the optimal dietary Cu level using the brokenline model. Dietary supplemental Cu level affected (P < 0.05) Cu contents in serum and liver and kidney monoamine oxidase (MAO) activity, but had no effects (P> 0.05) on the growth performance, Cu contents in heart, kidney, pancreas and spleen, Cu- and zinc-containing superoxide dismutase (CuZnSOD) activity and ceruloplasmin content in serum, CuZnSOD and cytochrome c oxidase (COX) activities and ceruloplasmin, CuZnSOD, MAO A, MAO B, COX 4I1 and COX 1 mRNA and protein expressions in the above tissues of broilers. As dietary supplemental Cu levels increased, Cu contents in serum and liver increased linearly (P < 0.05), but kidney MAO activity decreased linearly and quadratically (P < 0.05). The estimated dietary Cu requirement based on the fitted broken-line model (P = 0.035) of kidney MAO activity was 11.30 mg/kg. In conclusion, kidney MAO activity is a new and sensitive criterion to evaluate the dietary Cu requirement of broilers, and the dietary Cu requirement was 11.30 mg/kg for broilers fed the conventional corn-soybean meal diet from 1 to 21 d of age, which is higher than the current National Research Council (NRC) Cu requirement (8 mg/kg) of broilers. (C) 2021 Chinese Association of Animal Science and Veterinary Medicine. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co. Ltd.

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