A flexible assay strategy for non-glucose targets based on sulfhydryl-terminated liposomes combined with personal glucometer

文献类型： 外文期刊

第一作者： Nie, Dongxia

作者： Nie, Dongxia;Zhang, Zhiqi;Guo, Dakai;Huang, Qingwen;Zhao, Zhihui;Han, Zheng;Tang, Yupeng;Hu, Xiuli

作者机构：

关键词： Sulfhydryl-terminated liposomes; Chemical conjugation-based recognition; Magnetic NH2-Au@Fe3O4 nanoparticles; Non-glucose targets; Personal glucose meter

期刊名称：BIOSENSORS & BIOELECTRONICS （ 影响因子：10.618； 五年影响因子：9.323 ）

ISSN： 0956-5663

年卷期： 2021 年 175 卷

页码：

收录情况： SCI

摘要： The personal glucose meter (PGM) is one of the most successful point-of-care (POC) testing devices. It is simple, robust and inexpensive, but cannot be easily adapted to analytes other than glucose. We report a novel chemical conjugation-based assay strategy, using rational design of chemically-derivatized glucose-encapsulating liposomes, to repurpose a PGM, taking an important mycotoxin patulin as the model analyte. Sulfhydryl (-SH) was proposed for the first time as a specific functional group for efficient recognition of patulin. Multifunctional sulfhydryl-terminated glucose-encapsulating liposomes (G-LIP-SH) were synthesized in a simple, single step, which efficiently captured patulin by covalent bonding, and interacted strongly with NH2-Au@Fe3O4 nanoparticles. Magnetic removal of nanoparticles efficiently and selectively separated patulin-derivatized from underivatized G-LIP-SH, permitting the latter to be lysed and the released glucose measured by PGM. The PGM signal was inversely proportional to the patulin concentration, over the range of 0.1-50 ng mL(-1) (R-2 = 0.995) with a detection limit of 0.05 ng mL(-1) (S/N = 3). This approach overcame interference from endogenous glucose, other mycotoxins and metal ions, allowing the analysis of a wide range of sample matrices and showed high specificity, acceptable reproducibility, good accuracy and optimal applicability. Other derivatization chemistries will enable this approach to be adapted to analytes with a wide range of chemical structures, to facilitate development of rapid, portable, user-friendly and cost-effective assays applicable to diverse analytes and sample matrices.

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