Regulation Mechanism of Dopamine Receptor 1 in Low Temperature Response of Marsupenaeus japonicus

文献类型： 外文期刊

第一作者： Ren, Xianyun

作者： Ren, Xianyun;Bian, Xueqiong;Shao, Huixin;Jia, Shaoting;Yu, Zhenxing;Liu, Ping;Li, Jian;Li, Jitao;Ren, Xianyun;Bian, Xueqiong;Shao, Huixin;Jia, Shaoting;Yu, Zhenxing;Liu, Ping;Li, Jian;Li, Jitao;Bian, Xueqiong;Shao, Huixin;Yu, Zhenxing

作者机构：

关键词： Marsupenaeus japonicus; low temperature; RNA interference; DAD1; apoptosis

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.6； 五年影响因子：6.2 ）

ISSN： 1661-6596

年卷期： 2023 年 24 卷 20 期

页码：

收录情况： SCI

摘要： Dopamine receptors (DARs) are important transmembrane receptors responsible for receiving extracellular signals in the DAR-mediated signaling pathway, and are involved in a variety of physiological functions. Herein, the D1 DAR gene from Marsupenaeus japonicus (MjDAD1) was identified and characterized. The protein encoded by MjDAD1 has the typical structure and functional domains of the G-protein coupled receptor family. MjDAD1 expression was significantly upregulated in the gills and hepatopancreas after low temperature stress. Moreover, double-stranded RNA-mediated silencing of MjDAD1 significantly changed the levels of protein kinases (PKA and PKC), second messengers (cyclic AMP (cAMP), cyclic cGMP, calmodulin, and diacyl glycerol), and G-protein effectors (adenylate cyclase and phospholipase C). Furthermore, MjDAD1 silencing increased the apoptosis rate of gill and hepatopancreas cells. Thus, following binding to their specific receptors, G-protein effectors are activated by MjDAD1, leading to DAD1-cAMP/PKA pathway-mediated regulation of caspase-dependent mitochondrial apoptosis. We suggest that MjDAD1 is indispensable for the environmental adaptation of M. japonicus.

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