AcWRKY28 mediated activation of AcCPK genes confers salt tolerance in pineapple (Ananas comosus)

文献类型： 外文期刊

第一作者： Zhou, Qiao

作者： Zhou, Qiao;Luo, Tiantian;Mohammadi, Mohammad Aqa;Cai, Hanyang;Qin, Yuan;Wang, Lulu;Zhou, Qiao;Priyadarshani, Samaranayaka Vidana Gamage Nirosha;Cheng, Han;Luo, Tiantian;Wai, Myat Hnin;Mohammadi, Mohammad Aqa;Liu, Yanhui;Liu, Chang;Cai, Hanyang;Qin, Yuan;Qin, Yuan;Priyadarshani, Samaranayaka Vidana Gamage Nirosha;Qin, Rongjuan;Wang, Xiaomei;Liu, Yeqiang

作者机构：

关键词： Pineapple; AcWRKY; AcCPK; Transcription factor; Salt stress; ChIP

期刊名称：HORTICULTURAL PLANT JOURNAL （ 影响因子：5.7； 五年影响因子：5.4 ）

ISSN： 2095-9885

年卷期： 2024 年 10 卷 2 期

页码：

收录情况： SCI

摘要： Unfavorable environmental cues severely affect crop productivity resulting in significant economic losses to farmers. In plants, multiple regulatory genes, such as the WRKY transcription factor (TF) family, modulate the expression of defense genes. However, the role of the pineapple WRKY genes is poorly understood. Here, we studied the pineapple WRKY gene, AcWRKY28, by generating AcWRKY28 over-expressing transgenic pineapple plants. Overexpression of AcWRKY28 enhanced the salt stress resistance in transgenic pineapple lines. Comparative transcriptome analysis of transgenic and wild -type pineapple plants showed that "plant-pathogen interaction" pathway genes, including 9 calcium -dependent protein kinases (CPKs), were up-regulated in AcWRKY28 over-expressing plants. Furthermore, chromatin immunoprecipitation and yeast one -hybrid assays revealed AcCPK12, AcCPK3, AcCPK8, AcCPK1, and AcCPK15 as direct targets of AcWRKY28. Consistently, the study of AcCPK12 over-expressing Arabidopsis lines showed that AcCPK12 enhances salt, drought, and disease resistance. This study shows that AcWRKY28 plays a crucial role in promoting salt stress resistance by activating the expression of AcCPK genes.

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