miR-370-3p Inhibited the Proliferation of Sheep Dermal Papilla Cells by Inhibiting the Expression of SMAD4

文献类型： 外文期刊

第一作者： Fu, Jiaqi

作者： Fu, Jiaqi;Wang, Dan;Liu, Wenqing;Qi, Yu;Zhang, Caihong;Li, Huansong;Cai, Jinshun;Ji, Shuang;Sun, Fuliang;Wang, Dan;Qi, Yu;Zhang, Caihong;Zhang, Lichun

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关键词： miR-370-3p; SMAD4; dermal papilla cells; cell phenotype

期刊名称：CELLS （ 影响因子：5.2； 五年影响因子：6.1 ）

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年卷期： 2025 年 14 卷 10 期

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收录情况： SCI

摘要： The proliferation and maturation of hair follicles in follicular papilla cells are predominantly governed by miRNAs, which significantly influence the cell cycle, apoptosis, and proliferation. miR-370-3p has been associated with several biological processes and targets SMAD4, a crucial component in hair follicle development. Tissue expression profiling revealed significant differences in miR-370-3p levels between skin tissues of the two sheep breeds in January and October, as well as between tissues of the Xinji fine-wool sheep and Small-tail Han sheep. SMAD4 exhibited significant differences in tissue-specific expression in the heart, spleen, skin, lungs, and muscles from Xinji fine-wool sheep and Small-tail Han sheep. Bioinformatics analysis and dual-luciferase reporter assays validated the regulatory interaction between miR-370-3p and SMAD4. CCK-8 experiments demonstrated that miR-370-3p's targeting of SMAD4 suppressed cell growth. Cell cycle analysis demonstrated that miR-370-3p's targeting of SMAD4 influenced the cell cycle. Annexin V-FITC/PI dual labeling demonstrated that miR-370-3p's targeting of SMAD4 promoted cell apoptosis. RT-qPCR data demonstrated that miR-370-3p's targeting of SMAD4 elevated the expression of JUN, c-MYC, and TCF7L2 while suppressing beta-catenin expression. Western blot (WB) analysis demonstrated that miR-370-3p targeting of SMAD4 significantly promoted c-MYC expression while inhibiting CCND1, CCND2, and beta-catenin expression. miR-370-3p and SMAD4 exhibit spatiotemporal expression differences in sheep skin tissues, with widespread expression across various tissues. Furthermore, it confirmed that miR-370-3p targets SMAD4 to inhibit follicular papilla cell proliferation, promote apoptosis, and influence the cell cycle.

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