OsAMT1.1 Expression by Nitrate-Inducible Promoter of OsNAR2.1 Increases Nitrogen Use Efficiency and Rice Yield

文献类型： 外文期刊

第一作者： Jiang, Hongzhen

作者： Jiang, Hongzhen;Wang, Yamei;Lai, Liuru;Liu, Xintong;Miao, Changjian;Li, Xiaoyun;Tan, Jinfang;Chen, Jingguang;Liu, Ruifang;Gao, Zhenyu

作者机构：

关键词： OsAMT1; 1; OsNAR2; 1 promoter; nitrogen use efficiency; yield; rice

期刊名称：RICE SCIENCE （ 影响因子：4.8； 五年影响因子：5.5 ）

ISSN： 1672-6308

年卷期： 2023 年 30 卷 3 期

页码：

收录情况： SCI

摘要： Nitrate (NO3-) and ammonium (NH4+) are two main inorganic nitrogen (N) sources during crop growth. Here, we enhanced the expression of OsAMT1.1, which encodes a NH4+ transporter, using the NO3--inducible promoter of OsNAR2.1 and an ubiquitin promoter in transgenic rice plants. Under field condition of 120 kg /hm2 N, agronomic N use efficiency, N recovery efficiency and N transport efficiency, and grain yield of the pOsNAR2.1:OsAMT1.1 transgenic lines were increased compared with those of the wild type (WT) and the pUbi:OsAMT1.1 transgenic plants. Under 2.0 mmol/L NO3- + 0.5 mmol/L NH4+ and 0.5 mmol/L NO3- + 2.0 mmol/L NH4+ conditions of hydroponic culture, compared with the WT, both biomass and total N content were increased in the pOsNAR2.1:OsAMT1.1 transgenic lines. However, biomass was significantly reduced in pUbi:OsAMT1.1 transgenic plants under 0.5 mmol/L NO3- + 2.0 mmol/L NH4+ condition. The lines expressing pOsNAR2.1:OsAMT1.1 exhibited increased OsAMT1.1 expression and 15NH4+ influx in roots under both 2.0 mmol/L NO3- + 0.5 mmol/L NH4+ and 0.5 mmol/L NO3- + 2.0 mmol/L NH4+ conditions. Our study showed that expression of OsAMT1.1 can be promoted when driven by the OsNAR2.1 promoter, especially under high-level nitrate condition, leading to enhancement of NH4+ N

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