Okara protein extracted by alternating ultrasonic/alkali treatment and its improved physicochemical and functional properties

文献类型： 外文期刊

第一作者： Tang, Lu

作者： Tang, Lu;Liu, Xiaolin;Bai, Shiru;Zhao, Dan;Guo, Xuzhen;Zhu, Dandan;Su, Guiying;Fan, Bei;Wang, Bo;Zhang, Liang;Wang, Fengzhong

作者机构：

关键词： Okara; Protein; Alkali; Ultrasound; Functional properties; Emulsion stability

期刊名称：ULTRASONICS SONOCHEMISTRY （ 影响因子：9.7； 五年影响因子：9.1 ）

ISSN： 1350-4177

年卷期： 2024 年 111 卷

页码：

收录情况： SCI

摘要： Okara protein (OP) is a potential plant-based protein that is beneficial to human health. In this work, an alternating ultrasonic/alkali treatment method with non-continued cavitation and thermal energy output was used to extract protein (AUA-OP) from okara to enhance the functional properties of OP and improve the stability of OP-based emulsions. The purity of AUA-OP was greater than 80%. Compared with traditional (physicalassisted) alkali treatment, FTIR and SDS-PAGE revealed that AUA-OP retained the chemical structure of the protein, but the number of ultrasound-induced exposure sites increased, with increased fluorescence intensity, surface hydrophobicity, and absolute zeta-potential. After alternating ultrasonic/alkali treatment, the protein particles were looser and smaller. In addition, the water/oil holding capacity, EAI, and ESI of AUA-OP further increased. The viscosity of the AUA-OP-stabilized emulsion was also greater. Finally, a 28-day emulsion storage assay revealed that the AUA-OP-stabilized emulsion was stable with a relatively low droplet size and creaming index, indicating great potential for the development of stable protein-based emulsions.

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