Immunoprotective effects of DNA vaccine against Eimeria tenella based on EtAMA3 and EtRON2L2

文献类型： 外文期刊

第一作者： Chen, Xiangjie

作者： Chen, Xiangjie;Qiu, Xixi;Ni, Junli;Liao, Shenquan;Qi, Nanshan;Li, Juan;Lv, Minna;Lin, Xuhui;Cai, Haiming;Hu, Junjing;Song, Yongle;Yin, Lijun;Zhu, Yibin;Zhang, Jianfei;Zhang, Haoji;Sun, Mingfei

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关键词： Eimeria tenella; DNA vaccine; Immunoprotective efficacy

期刊名称：VETERINARY PARASITOLOGY （ 影响因子：2.6； 五年影响因子：2.5 ）

ISSN： 0304-4017

年卷期： 2024 年 327 卷

页码：

收录情况： SCI

摘要： Eimeria tenella is the most pathogenic and harmful intestinal parasitic protozoan. Recombinant DNA vaccines open options for promising strategies for preventing avian coccidiosis, replacing chemical drugs and live oocyst vaccines. Two important antigenic proteins, EtAMA3 (also known as SporoAMA1) and EtRON2L2, act together to promote the invasion of E. tenella sporozoites. In this study, a recombinant DNA vaccine, designated pcDNA3.1 (+)-AR, was constructed based on EtAMA3DII, EtRON2L2D3, and EtRON2L2D4. Chickens were intramuscularly immunized with different doses (25, 50, or 100 mu g) of pcDNA3.1(+)-AR to evaluate its immunoprotective effects in vivo. The chickens in the 50 mu g and 100 mu g groups had higher cytokine concentrations (interleukin 2, interferon -gamma, and interleukin 10), and lesion scores (81.9% and 67.57%, respectively) and relative oocyst production (47% and 19%, respectively) reduced compared with the unchallenged group, indicating partial protection against E. tenella. These results suggest that pcDNA3.1(+)-AR is a promising vaccine candidate against avian coccidiosis.

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