The F204S mutation in adrenodoxin oxidoreductase drives salinomycin resistance in Eimeria tenella

文献类型： 外文期刊

第一作者： Sun, Pei

作者： Sun, Pei;Xie, Fujie;Chen, Linlin;Gao, Yang;Zhang, Ning;Hao, Zhenkai;Suo, Jingxia;Suo, Xun;Liu, Xianyong;Sun, Pei;Xie, Fujie;Chen, Linlin;Gao, Yang;Zhang, Ning;Hao, Zhenkai;Suo, Jingxia;Suo, Xun;Liu, Xianyong;Sun, Pei;Xie, Fujie;Chen, Linlin;Gao, Yang;Zhang, Ning;Hao, Zhenkai;Suo, Jingxia;Suo, Xun;Liu, Xianyong;Wang, Chaoyue;Zhang, Yuanyuan;Zhang, Yuanyuan;Tang, Xinming;Hu, Dandan;Yu, Yonglan

作者机构：

关键词： Eimeria tenella; salinomycin; drug resistance; point mutation

期刊名称：VETERINARY RESEARCH （ 影响因子：3.5； 五年影响因子：4.0 ）

ISSN： 0928-4249

年卷期： 2024 年 55 卷 1 期

页码：

收录情况： SCI

摘要： Salinomycin is a polyether ionophore widely used for the treatment of coccidiosis in poultry. However, the emergence of coccidia strains resistant to salinomycin presents challenges for control efforts, and the mechanisms underlying this resistance in Eimeria remain inadequately understood. In this study, 78 stable salinomycin-resistant strains were generated through experimental evolution approaches. Whole-genome sequencing of salinomycin-resistant Eimeria tenella isolates revealed single nucleotide polymorphisms (SNPs), with 12 candidate genes harboring nonsynonymous mutations identified. To confirm the candidate gene responsible for conferring salinomycin resistance, we leveraged reverse genetic strategies and identified a key amino acid substitution (F204S) in adrenodoxin oxidoreductase (EtADR), which markedly reduced susceptibility to salinomycin. Our results elucidate the complex interactions among salinomycin resistance, parasite fitness, point mutations, and the structure of EtADR, laying the foundation for future studies on drug resistance in Eimeria and contributing to the development of targeted control strategies.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序