文献类型： 外文期刊

第一作者： Qi Yu

作者： Qi Yu;Gao Xiao-Jin;Jiang Na;Guo Li;Xie Shou-An;Lv Shu-Jie;Cheng Hong-Gang;Gao Xiao-Jin;Guo Li;Cheng Hong-Gang

作者机构：

关键词： Agrilus zanthoxylumi; chemosensory protein; gene cloning; bioinformatics analysis; RT-qPCR

期刊名称：JOURNAL OF ENTOMOLOGICAL SCIENCE （ 影响因子：0.7； 五年影响因子：0.9 ）

ISSN： 0749-8004

年卷期： 2024 年 59 卷 3 期

页码：

收录情况： SCI

摘要： Chemosensory proteins (CSPs) are widely distributed in insect tissues and are involved in olfactory and non-olfactory functions. In this study, based on the transcriptome data of Agrilus zanthoxylumi Hou (Coleoptera: Buprestidae), the AzanCSP4 of was cloned by RT-PCR and bioinformatically analyzed, and RT-qPCR was conducted to analyze their expression levels of AzanCSP4 in different genders and tissues (head, thorax, abdomen, leg and wing). Sequence analysis showed that AzanCSP4 had an open reading frame (ORF) length of 366 bp, encoding 121 amino acids with an estimated molecular weight 13.96 kD. The encoded protein had no transmembrane domain, and the signal peptide was located in the position 1-15 at the N-terminal of the amino acid sequence. Sequence alignment revealed that AzanCSP4 had four conserved cysteines. Phylogenetic analysis revealed that the AzanCSP4 and AmalCSP6 from Agrilus mali Matsumura (Coleoptera: Buprestidae) were closely clustered into the same clade. RT-qPCR results showed that AzanCSP4 of A. zanthoxylumi was expressed in different tissues of both male and female adults, and the expression in the same tissue was greater in female adults than in male adults. The expression of AzanCSP4 in the head of female adults was significantly higher than that in other tissues of male and female adults. This study provides a theoretical basis for further research on the function of AzanCSP4, especially on the chemical communication mechanism in A. zanthoxylumi.

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