Establishment of a DNA Barcoding Database for Legume and Grass Species Identification

文献类型： 外文期刊

第一作者： Li, Yongqing

作者： Li, Yongqing;La, Zunongjiang A. Bu;Cao, Lijun;Zhao, Shengguo

作者机构：

关键词： DNA barcoding; Forages; MatK; RbcL; Universal sequences

期刊名称：LEGUME RESEARCH （ 影响因子：0.669； 五年影响因子：0.762 ）

ISSN： 0250-5371

年卷期： 2022 年 45 卷 6 期

页码：

收录情况： SCI

摘要： Background: DNA barcoding, an emerging approach, is being widely used to accurately and quickly identify species using conserved DNA sequences. Methods: Herein we designed seven universal primers for matK (matK1, matK2, matK3 and matK4), rbcL, psbA-trnH and ITS based on their nucleotide sequences in GenBank to amplify 40 species of leguminosae and grass forages. Sequence alignment was performed using MEGA 5.0 and haplotype and mutation sites were analyzed with DnaSP 5.10. PCR amplification efficiency on using the primers designed for psbA-trnH and ITS was relatively low, making these sequences unsuitable for DNA barcoding. Further, we optimized target fragment amplification conditions for all 40 species analyzed in this study. On purifying, sequencing and analysing amplification products, we selected 5' - and 3' -end conserved fragments in four marked fragments. Result: Sequences of each maker loci showed that there were 12, 17 and 6 haplotypes of matK1, matK2 and matK3, respectively and 13 of rbcL. Based on these haplotypes of matK1, matK2, matK3 and rbcL, we established a DNA barcoding database for 20 forage species.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序