Research on mixed sample test for the rapid detection of rice stripe virus and southern rice black-streaked dwarf virus in vector

文献类型： 外文期刊

第一作者： Du, Linlin

作者： Du, Linlin;Li, Tao;Wang, Yunyue;Du, Linlin;Li, Tao;Li, Chenyang;Lan, Ying;Lin, Feng;Zhou, Yijun;Zhou, Tong;Guo, Wei;Xu, Kai;Kundu, Jiban Kumar;Du, Linlin;Zhou, Tong

作者机构：

关键词： Mixed sample test; RSV; SRBSDV; Sentinel surveillance; SBPH

期刊名称：VIROLOGY （ 影响因子：2.4； 五年影响因子：2.5 ）

ISSN： 0042-6822

年卷期： 2025 年 604 卷

页码：

收录情况： SCI

摘要： Rice is a staple food in East and Southeast Asian countries, rice disease surveillance and warning is particularly important. Rice stripe disease and southern rice black-streaked dwarf virus disease are the insect-borne viral diseases that easily break out and quickly become epidemic. It is important to improve the efficiency of the virus detection method. In this work, a colloidal gold strip assay (CGSA) was used in mixed samples to detect rice stripe virus (RSV) and southern rice black-streaked dwarf virus (SRBSDV) in small brown planthoppers (SBPH) and white-backed planthoppers (WBPH), respectively. The results show that RSV could be detected in 9, 19 and 49 SBPHs mixed with a single positive SBPH sample, and SRBSDV could be detected in 9 WBPHs mixed with a single positive WBPH sample. The mixed sample detection technology presented in this article can significantly reduce the cost of detection and fulfil the requirements for large-scale, real-time virus surveillance in sentinel surveillance.

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