EGR1 mRNA expression levels and polymorphisms are associated with slaughter performance in chickens

文献类型： 外文期刊

第一作者： Ye, Mao

作者： Ye, Mao;Ye, Chutian;Guo, Lijin;Fan, Zhexia;Ma, Xuerong;Liu, Aijun;Liang, Weiming;Chen, Shuya;Fang, Cheng;Zhang, Xiquan;Luo, Qingbin;Ye, Mao;Chao, Xiaohuan;Ye, Chutian;Guo, Lijin;Fan, Zhexia;Ma, Xuerong;Liu, Aijun;Liang, Weiming;Chen, Shuya;Fang, Cheng;Zhang, Xiquan;Luo, Qingbin;Ye, Mao;Chao, Xiaohuan;Ye, Chutian;Guo, Lijin;Fan, Zhexia;Ma, Xuerong;Liu, Aijun;Liang, Weiming;Chen, Shuya;Fang, Cheng;Zhang, Xiquan;Luo, Qingbin;Ye, Mao;Chao, Xiaohuan;Ye, Chutian;Guo, Lijin;Fan, Zhexia;Ma, Xuerong;Liu, Aijun;Liang, Weiming;Chen, Shuya;Fang, Cheng;Zhang, Xiquan;Luo, Qingbin

作者机构：

关键词： Early growth response protein 1 ( EGR1 ); Polymorphism; Slaughter performance; Association analysis; Skeletal muscle

期刊名称：POULTRY SCIENCE （ 影响因子：4.2； 五年影响因子：4.5 ）

ISSN： 0032-5791

年卷期： 2025 年 104 卷 1 期

页码：

收录情况： SCI

摘要： With the implementation of the policy of "centralized slaughtering and chilled to market" and the development of the livestock processing industry, numerous researchers have begun to focus on the selection and breeding of broilers bred for slaughter. The selection of breeds with excellent slaughtering performance and high meat production performance has become one of the most important selective breeding goals. In our previous study, we conducted transcriptome sequencing on chicken breast tissues with high and low breast muscle rates and found higher early growth response protein 1 (EGR1) expression in breast tissues with a low breast muscle ratio, thus hypothesizing that the EGR1 gene is involved in the growth and development process of chicken muscle tissues. Therefore, we analyzed the gene functions and polymorphisms of EGR1 to investigate its association with slaughter traits. We used various experimental methods, including RT-qPCR, Cell Counting Kit 8, 5-ethynyl-2 ' deoxyuridine, western blot, flow cytometry, and immunofluorescence, to validate EGR1's role in chicken primary myoblasts. The results of our functional validation experiments indicate that EGR1 is highly expressed in breast tissues with a low breast muscle content and plays a key role in regulating of muscle growth and development by promoting proliferation and inhibiting the differentiation of chicken primary myoblasts. In addition, we explored the relationship between the EGR1 gene polymorphisms and slaughter traits using mixed linear models for the first time. In a population of Jiangfeng M3 lineage partridge chickens, we identified 4 EGR1 single-nucleotide polymorphisms, 2 of which were significantly associated with slaughter traits, including live weight, slaughter weight, semi-eviscerated weight, eviscerated weight, leg weight, wing weight, and breast muscle rate. In summary, ectopic expression of EGR1 promotes the proliferation and differentiation of chicken primary myoblasts. In addition, polymorphisms in EGR1 were associated with slaughter performance, providing a potential basis for further utilization of EGR1 as a breeding marker.

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