Development of an indirect ELISA method based on the VP1 protein for detection of IgG antibodies against porcine sapelovirus

文献类型： 外文期刊

第一作者： Liu, Jiajia

作者： Liu, Jiajia;Li, Benqiang;Tao, Jie;Cheng, Jinghua;Shi, Ying;Qiao, Changtao;Shen, Xiaohui;Liu, Huili;Liu, Jiajia

作者机构：

期刊名称：CANADIAN JOURNAL OF VETERINARY RESEARCH-REVUE CANADIENNE DE RECHERCHE VETERINAIRE （ 影响因子：1.1； 五年影响因子：1.3 ）

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年卷期： 2023 年 87 卷 3 期

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收录情况： SCI

摘要： Porcine sapelovirus (PSV) is a newly emerging enterovirus that is widely prevalent in China. Since there is no clinical serological testing for PSV, the objective of this study was to develop an indirect enzyme-linked immunosorbent assay (i-ELISA) for detection of PSV immunoglobulin G (IgG) antibody in pigs. A PSV strain, named SHPD202148, was first isolated from the fecal samples of piglets. Its structural protein, VP1, was prokaryotic- expressed in the pET expression system, followed by purification. Using the recombinant protein with reactogenicity as coating antigen, an i- ELISA, characterized by high sensitivity and specificity, had a detection limit at 1:12 800 dilution with a determined cutoff value of 0.352. Finally, field sera collected from different pig herds were tested in parallel by the serum neutralization ( SN) test. The result showed that 126 samples were positive and 36 were negative, with an agreement of 97.0% in both cases. This i-ELISA can be used as an alternative serological test for detecting antibodies against PSV in blood serum.

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