Flagellar rotor protein FliG is involved in the virulence of avian pathogenic Escherichia coli

文献类型： 外文期刊

第一作者： Yin, Lei

作者： Yin, Lei;Shen, Xuehuai;Zhang, Danjun;Zhao, Ruihong;Dai, Yin;Hu, Xiaomiao;Zhou, Xueli;Hou, Hongyan;Pan, Xiaocheng;Qi, Kezong

作者机构：

关键词： APEC; fliG; Motility; Adhesion; Invasion; Virulence

期刊名称：MICROBIAL PATHOGENESIS （ 影响因子：3.738； 五年影响因子：3.664 ）

ISSN： 0882-4010

年卷期： 2021 年 160 卷

页码：

收录情况： SCI

摘要： Avian pathogenic Escherichia coli (APEC), a type of extraintestinal pathogenic E. coli, causes avian colibacillosis, a disease of significant economic importance to poultry producers worldwide, which is characterized by systemic infection. However, the pathogenesis of avian pathogenic E. coli strains is not well defined. Here, the role of a flagellar rotor protein encoded by the fliG gene of avian pathogenic E. coli strain AE17 was investigated. To study the role of FliG in the pathogenicity of APEC, fliG mutant and complemented strains were constructed and characterized. The inactivation of fliG had no effect on APEC growth, but significantly reduced bacterial motility. Compared with the wild type, the fliG mutant was highly attenuated in a chick infection model and showed severe defects in its adherence to and invasion of chicken embryo fibroblast DF-1 cells. The fliG mutant also showed reduced resistance to serum in chicks. The expression of the inflammatory cytokines interleukin 1 beta (IL1 beta), IL6, and IL8 was reduced in HD-11 macrophages infected with the fliG mutant strain compared with their expression in the wild-type strain. These results demonstrate that the FliG contributes to the virulence of APEC.

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