Enzymatically synthesized gamma-[Glu]((n >= 1))-Gln as novel calcium-binding peptides to deliver calcium with enhanced bioavailability

文献类型： 外文期刊

第一作者： Xiang, Huan

作者： Xiang, Huan;Huang, Hui;Hu, Xiao;Li, Laihao;Sun-Waterhouse, Dongxiao;Tang, Runmei;Xiong, Jian;Cui, Chun;Cui, Chun;Sun-Waterhouse, Dongxiao;Waterhouse, Geoffrey I. N.

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关键词： gamma-[Glu]((n >= 1))-Gln; gamma-[Glu]((n >= 1))-Gln-Ca chelate; Coordination mechanism; Caco-2 cells; Calcium bioavailability

期刊名称：FOOD CHEMISTRY （ 影响因子：9.231； 五年影响因子：8.795 ）

ISSN： 0308-8146

年卷期： 2022 年 387 卷

页码：

收录情况： SCI

摘要： Novel gamma-[Glu]((n >= 1))-Gln-Ca chelates were prepared by using gamma-[Glu]((n >= 1))-Gln (mixture peptides containg gamma-Glu-Gln, gamma-Glu-Glu-Gln, gamma-Glu-Glu-Glu-Gln, gamma-Glu-Glu-Glu-Glu-Gln, gamma-Glu-Glu-Glu-Glu-Glu-G1n) which were synthesized by glutaminase. Based on single factor and orthogonal array experiments, the optimal calcium-chelation conditions were as follows: 30 min chelation at 70 degrees C; peptide concentration, 0.15 g/mL; mixture peptides-tocalcium mass ratio, 25:1. The particle size of chelates was 743.6 +/-( )64.2 nm and the chelates appeared stable neutral molecular entities with 91.33% of chelation rate and 35.2 mg/g of calcium, also with higher thermostability. Chelated calcium ions might be surrounded by coordination bonds linking to two or more connecting points: oxygen atoms (carboxyl oxygen, hydroxyl oxygen and oxygen of peptide bonds) and nitrogen atoms (amino nitrogen). The chelates could be used for food/nutraceutical applications, as no toxicity at 0.125-10 mg/mL and enhanced efficiency in calcium release (releasing percentage over 85% at pH 8.0), transport and uptake compared with CaCl2 were detected in Caco-2 cells.

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