Identification and subcellular localization of the chaperonin NbCCT(3 in Nosema bombycis

文献类型： 外文期刊

第一作者： Xiao, Shengyan

作者： Xiao, Shengyan;Sun, Jiancheng;Chen, Ying;Wang, Runpeng;Shen, Zhongyuan;Shen, Zhongyuan;Xiao, Shengyan;Sun, Jiancheng;Wang, Xiaowei;Zhu, Feng

作者机构：

关键词： Microsporidia; Nosema bombycis; Chaperonin; NbCCT(3; Immunolocalization

期刊名称：GENE （ 影响因子：2.4； 五年影响因子：2.8 ）

ISSN： 0378-1119

年卷期： 2025 年 941 卷

页码：

收录情况： SCI

摘要： Nosema bombycis, the causative agent of pebrine disease, poses a significant threat to the silkworm industry due to its negative impact on silkworm health and productivity. The chaperonin-containing tailless complex polypeptide (CCT) plays a crucial role in protein folding, and its (3 subunit (CCT(3) is essential for the proper folding of cytoskeletal proteins, such as actin and tubulin. In this study, we cloned and expressed the NbCCT beta gene from N. bombycis and generated polyclonal anti-NbCCT(3 antibodies to further assess NbCCT(3. Immunofluorescence subcellular localization studies revealed that NbCCT(3 is primarily located in the cytoplasm and shows extensive co-localization with NbCCTS and NbCCT theta during the proliferation stage of the pathogen, suggesting its involvement in key cellular processes. Moreover, NbCCT(3 was found to co-localize with actin and tubulin, indicating a role in cytoskeletal assembly. Notably, NbCCT beta expression increased with infection duration, and RNA interference effectively reduced its expression, indicating its importance in N. bombycis survival and proliferation. These findings highlight NbCCT(3 as a potential target for interventions aimed at mitigating pebrine disease, providing a foundation for future strategies to protect the silkworm industry.

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