Identification of the arl1 locus controlling leaf rolling and its application in maize breeding

文献类型： 外文期刊

第一作者： Yang, Meng

作者： Yang, Meng;Huang, Aihua;Wen, Renlai;Tian, Shuyun;Mo, Runxiu;Zhai, Ruining;Gong, Xue;He, Xueyin;Li, Faqiao;Huang, Kaijian;Zou, Chenglin;Yang, Xiaohong;Chen, Wenkang;Yang, Xiaohong;Chen, Wenkang;Chen, Wenkang

作者机构：

关键词： Leaf rolling; Bulk segregation analysis mapping; Photosynthetic rate; High-density planting tolerance; Maize breeding

期刊名称：MOLECULAR BREEDING （ 影响因子：3.0； 五年影响因子：3.0 ）

ISSN： 1380-3743

年卷期： 2025 年 45 卷 1 期

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收录情况： SCI

摘要： Increasing planting density is one of the most important strategies for generating higher maize yields. Moderate leaf rolling decreases mutual shading of leaves and increases the photosynthesis of the population and hence increases the tolerance for high-density planting. Few genes that control leaf rolling in maize have been identified, however, and their applicability for breeding programs remains unclear. Here we identified a maize abaxially rolled leaf1 (arl1) mutant with extreme abaxially rolled leaves and found that the size of the bulliform cells within the adaxial leaf blade surface increased in the arl1 mutant. Bulk segregation analysis mapping in an F2 population derived from a single cross between arl1 and inbred line Gui18421 with normal leaves identified the arl1 locus on chromosome 2. Sequential fine-mapping delimited the arl1 locus to a 233.56-kb genomic interval containing three candidate genes. Sequence alignment between arl1 and Gui18421 identified an 8-bp insertion in the coding region of Zm00001eb082500, which led to a frame shift causing premature transcription termination in arl1 mutant. Meanwhile, both deep sequencing and Sanger sequencing showed that Zm00001eb082520 was present in Gui18421 but was absent in arl1. A pair of near isogenic lines (NILs) carrying the Gui18421 allele (NILGui18421) and the arl1 allele (NILarl1) were developed, and the leaves of NILarl1 plants had greater light transmission and photosynthetic rate in the middle and lower canopy than did those of NILGui18421 plants under high-density planting. Furthermore, NILarl1 had a higher seed setting rate, more kernels per ear, and an increased kernel weight per ear than NILGui18421, and the grain yield of NILarl1 was not affected as the planting density increased, suggesting that the arl1 locus can be used for genetic improvement of high-density planting tolerance. Taken together, the identification of arl1 and evaluation of yield-related traits for NILGui18421 and NILarl1 provide an excellent target for future maize improvement.

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