Clade V MLO Genes Are Negative Modulators of Cucumber Defence Response to Meloidogyne incognita

文献类型： 外文期刊

第一作者： Xie, Xiaoxiao

作者： Xie, Xiaoxiao;Ling, Jian;Dong, Shaoyun;Zhai, Mingjuan;Lu, Junru;Zhao, Jianlong;Yang, Xueyong;Dong, Xin;Li, Yan;Mao, Zhenchuan;Zhang, Shengping;Xie, Bingyan;Xie, Xiaoxiao;Visser, Richard G. F.;Bai, Yuling;Xie, Xiaoxiao

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关键词： CRISPR; cucumber; Meloidogyne incognita; MLO; susceptibility genes; VIGS

期刊名称：MOLECULAR PLANT PATHOLOGY （ 影响因子：4.9； 五年影响因子：5.6 ）

ISSN： 1464-6722

年卷期： 2025 年 26 卷 4 期

页码：

收录情况： SCI

摘要： Cucumber production is seriously constrained by Meloidogyne incognita. Because no resistance resources to the pathogen have been reported, disabling susceptibility genes may represent a novel breeding strategy to introduce resistance against this nematode in cucumber. Here, we studied the clade V MLO genes for their involvement in the interaction between cucumber and M. incognita. Our results showed that Arabidopsis clade V MLO mutants were resistant to M. incognita. Cucumber has three clade V MLO genes, CsaMLO1, CsaMLO8 and CsaMLO11, with upregulated expression upon inoculation with M. incognita. Heterologous overexpression of CsaMLO1, CsaMLO8 and CsaMLO11 in Arabidopsis mutants restored susceptibility to varying degrees. Silencing and knockout of individual clade V MLO genes in cucumber reduced susceptibility to M. incognita. The cucumber CRISPR mutants produced similar fruits as the wild type (WT) did. Although the yields of two single mutants (M11(1) and M11(2)) and two double mutants (M8(1)M11(1) and M8(1)M11(2)) were reduced compared to WT, the yields of M8(1) and M8(2) were not decreased. In summary, clade V MLO genes function as susceptibility genes for M. incognita in cucumber. Among them, CsaMLO8 may be the most promising candidate for M. incognita resistance breeding in cucumber.

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