Embryo Rescue Breeding of New Cold-Resistant, Seedless Grapes

文献类型： 外文期刊

第一作者： Chu, Yannan

作者： Chu, Yannan;Li, Min;Li, Ruonan;Zhang, Kangzhuang;Qiu, Pengpeng;Yuan, Xiaojian;Han, Yulei;Liu, Xinyu;Xu, Yan;Liu, Guotian;Chu, Yannan;Li, Min;Li, Ruonan;Zhang, Kangzhuang;Qiu, Pengpeng;Yuan, Xiaojian;Han, Yulei;Liu, Xinyu;Xu, Yan;Liu, Guotian;Chu, Yannan

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关键词： grape; embryo rescue breeding; seedless traits; cold resistance; molecular marker

期刊名称：HORTICULTURAE （ 影响因子：3.1； 五年影响因子：3.4 ）

ISSN：

年卷期： 2023 年 9 卷 9 期

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收录情况： SCI

摘要： Seedlessness in grapes is much appreciated by consumers and especially in cultivars consumed either as table grapes or as raisins. In many parts of the world, low temperature is the main environmental stress limiting grape production. In this study, stenospermocarpic (seed abortion) cultivars were selected as the female parents while seeded cold-resistant cultivars were selected as the male parents to develop new cold-resistant seedless grapes using embryo rescue technology, which has previously been shown to be a highly efficient way of breeding seedless grapes. Here, we report optima in genotype, sampling time, and culture medium for the embryo rescue of 14 hybrid combinations. Our results indicate that the embryo development rate (39.9%) and the seedlings rate (21.5%) were highest among the 14 crosses when 'Ruby Seedless' was used as the female parent and 'Beibinghong' was used as the male parent. The best sampling times for 'Yuehong Wuhe', 'Ruby Seedless', and 'Melissa seedless' were 37, 55, and 52 days after flowering, respectively. Embryo rescue efficiency was highest when the sucrose concentration for seedlings was maintained at about 1.0%. Using molecular markers, we detected 91 hybrids with seedless traits and 18 hybrids with cold resistance traits.

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