文献类型： 外文期刊

第一作者： Wang Chao-nan

作者： Wang Chao-nan;Luan Fei-shi;Liu Hong-yu;Liu Shi;Davis, Angela R.;Zhang Qi-an;Dai Zu-yun

作者机构：

关键词： watermelon; fine mapping; flesh color; QTL; transcriptome

期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：2.848； 五年影响因子：2.979 ）

ISSN： 2095-3119

年卷期： 2021 年 20 卷 8 期

页码：

收录情况： SCI

摘要： The color of watermelon flesh is an important trait determined by a series of carotenoids. Herein, we used Cream of Saskatchewan (pale yellow flesh) and PI 186490 (white flesh) as parental materials for an F2 segregation and initial mapping using the bulked segregant analysis sequencing (BSA-seq) strategy. The BSA results revealed a flesh colorrelated QTL that spans approximately 2.45 Mb on chromosome 6. This region was preliminarily positioned in a 382-kb segment, and then narrowed down into a 66.8-kb segment with 1 260 F2 individuals. A total of nine candidate genes were in the fine mapping interval, but only Cla007528 (encoding chlorophyllase) had non-synonymous mutations and was significantly expressed between the parental materials throughout flesh development. We also checked the expression patterns of the carotenoid metabolic pathway genes based on RNA-seq data and qRT-PCR validation. Three genes in the xanthophyll cycle (ClCHYB, ClNCED-1 and ClNCED-7) exhibited differential expression patterns between the two parental lines at different flesh color formation stages. ClPSY1, ClPDS, ClZDS, ClCHXE, ClCRTISO and ClLCYB also exhibited clearly different expression patterns accompanied by carotenoid accumulation.

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