Combining Physio-Biochemical Characterization and Transcriptome Analysis Reveal the Responses to Varying Degrees of Drought Stress in Brassica napus L.

文献类型： 外文期刊

第一作者： Fang, Shuai

作者： Fang, Shuai;Zhao, Peimin;Tan, Zengdong;Peng, Yan;Xu, Lintang;Jin, Yutong;Guo, Liang;Yao, Xuan;Fang, Shuai;Zhao, Peimin;Tan, Zengdong;Xu, Lintang;Jin, Yutong;Guo, Liang;Yao, Xuan;Peng, Yan;Guo, Liang;Wei, Fang;Wei, Fang;Guo, Liang

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关键词： Brassica napus; physio-biochemical characterization; transcriptome; drought tolerance; BnaCIPK6

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：6.208； 五年影响因子：6.628 ）

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年卷期： 2022 年 23 卷 15 期

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收录情况： SCI

摘要： Brassica napus L. has become one of the most important oil-bearing crops, and drought stress severely influences its yield and quality. By combining physio-biochemical characterization and transcriptome analysis, we studied the response of B. napus plants to different degrees of drought stress. Some physio-biochemical traits, such as fresh weight (FW), dry weight (DW), abscisic acid (ABA) content, net photosynthetic rate (Pn), stomatal conductance (g(s)), and transpiration rate (Tr), were measured, and the total content of the epidermal wax/cutin, as well as their compositions, was determined. The results suggest that both stomatal transpiration and cuticular transpiration are affected when B. napus plants are subjected to varying degrees of drought stress. A total of 795 up-regulated genes and 1050 down-regulated genes were identified under severe drought stress by transcriptome analysis. Gene ontology (GO) enrichment analysis of differentially expressed genes (DEGs) revealed that the up-regulated genes were mainly enriched in the stress response processes, such as response to water deprivation and abscisic acid, while the down-regulated genes were mainly enriched in the chloroplast-related parts affecting photosynthesis. Moreover, overexpression of BnaA01.CIPK6, an up-regulated DEG, was found to confer drought tolerance in B. napus. Our study lays a foundation for a better understanding of the molecular mechanisms underlying drought tolerance in B. napus.

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