An inducible CRISPR activation tool for accelerating plant regeneration

文献类型： 外文期刊

第一作者： Zhang, Cuimei

作者： Zhang, Cuimei;Xu, Yujun;Zhou, Yangyan;Liu, Qikun;Tang, Yajun;Yuan, Haidi;Zhou, Junhui;Tang, Shanjie;Chen, Lei;Li, Tong;Zhang, Shuaibin;Deng, Xian;Cao, Xiaofeng;Song, Xianwei;Wang, Jianli;Wen, Hongyu;Jiang, Wenbo;Pang, Yongzhen;Deng, Xian;Song, Xianwei

作者机构：

关键词： inducible CRISPR activation; morphogenic gene; genetic transformation; plant regeneration

期刊名称：PLANT COMMUNICATIONS （ 影响因子：10.5； 五年影响因子：10.5 ）

ISSN： 2590-3462

年卷期： 2024 年 5 卷 5 期

页码：

收录情况： SCI

摘要： The inducible CRISPR activation (CRISPR-a) system offers unparalleled precision and versatility for regulating endogenous genes, making it highly sought after in plant research. In this study, we developed a chemically inducible CRISPR-a tool for plants called ER-Tag by combining the LexA-VP16-ER inducible system with the SunTag CRISPR-a system. We systematically compared different induction strategies and achieved high efficiency in target gene activation. We demonstrated that guide RNAs can be multiplexed and pooled for large-scale screening of effective morphogenic genes and gene pairs involved in plant regeneration. Further experiments showed that induced activation of these morphogenic genes can accelerate regeneration and improve regeneration efficiency in both eudicot and monocot plants, including alfalfa, woodland strawberry, and sheepgrass. Our study expands the CRISPR toolset in plants and provides a powerful new strategy for studying gene function when constitutive expression is not feasible or ideal.

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