Interferon regulatory factor-2 in orange-spotted grouper (Epinephelus coioides): Gene, inductive expression pattern and subcellular localization

文献类型: 外文期刊

第一作者: Shi, Yan

作者: Shi, Yan;Yin, Jing-Kui;Zhu, Xin-Ping;Chen, Kun-Ci;Pan, De-Bo;Zhao, Zhe;Zhu, Xin-Ping;Gui, Jian-Fang

作者机构:

关键词: Interferon regulator factor-2 (IRF-2);Epinephelus coioides;Genomic structure;Inductive expression;Subcellular localization

期刊名称:COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY ( 影响因子:2.231; 五年影响因子:2.215 )

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收录情况: SCI

摘要: Interferon regulatory factor 2 (IRF-2) is a multifunctional transcription factor which exhibits both transcriptional activating and repressing activities in the IRF family. In this study, we report an IRF-2 gene isolated from orange-spotted grouper (Epinephelus coioides). The 1854 bp full-length cDNA sequence of EcIRF-2 has been cloned, encoding a putative peptide of 336 amino acids which is highly consistent with the feature of IRF family members. The genomic fragment of EcIRF-2 contains nine exons and eight introns, spanning over approximate 8.8 kb. The expression of EcIRF-2 gene was detected in various tissues of healthy orange-spotted grouper and in four tissues after being challenged with poly I:C or LPS. EcIRF-2 gene is ubiquitously expressed in various healthy fish tissues and is up-regulated in vivo in response to poly I:C or LPS. Subcellular localization analysis of EcIRF-2 suggests it is an intranuclear protein in the fish cells. We believe this research is the first report of fish IRF-2 protein localization. The results in this research establish the base for further study of function mechanism of IRF family members in orange-spotted grouper.

分类号: Q5

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