Identification of L11L and L7L as virulence-related genes in the African swine fever virus genome
文献类型： 外文期刊
第一作者： Fan, Jiaqi
作者： Fan, Jiaqi;Wang, Fengjie;Zhou, Xintao;Hu, Rongliang;Fan, Jiaqi;Wang, Fengjie;Miao, Faming;Zhang, Han;Jiang, Yiqian;Qi, Yu;Zhang, Yanyan;Hui, Lili;Zhang, Dan;Yue, Huixian;Zhou, Xintao;Li, Qixuan;Wang, Yu;Chen, Teng;Hu, Rongliang;Fan, Jiaqi;Wang, Fengjie;Miao, Faming;Zhang, Han;Jiang, Yiqian;Qi, Yu;Zhang, Yanyan;Hui, Lili;Zhang, Dan;Yue, Huixian;Zhou, Xintao;Li, Qixuan;Wang, Yu;Chen, Teng;Hu, Rongliang;Zhang, Jingyuan
作者机构：
关键词： African swine fever virus; L7L; L11L; virulence; recombinant virus
期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：5.2； 五年影响因子：6.2 ）
ISSN：
年卷期： 2024 年 15 卷
页码：
收录情况： SCI
摘要： Introduction: African swine fever (ASF) is an infectious disease that causes considerable economic losses in pig farming. The agent of this disease, African swine fever virus (ASFV), is a double-stranded DNA virus with a capsid membrane and a genome that is 170-194 kb in length encoding over 150 proteins. In recent years, several live attenuated strains of ASFV have been studied as vaccine candidates, including the SY18 Delta L7-11. This strain features deletion of L7L, L8L, L9R, L10L and L11L genes and was found to exhibit significantly reduced pathogenicity in pigs, suggesting that these five genes play key roles in virulence. Methods: Here, we constructed and evaluated the virulence of ASFV mutations with SY18 Delta L7, SY18 Delta L8, SY18 Delta L9, SY18 Delta L10, and SY18 Delta L11L. Results: Our findings did not reveal any significant differences in replication efficiency between the single-gene deletion strains and the parental strains. Pigs inoculated with SY18 Delta L8L, SY18 Delta L9R and SY18 Delta L10L exhibited clinical signs similar to those inoculated with the parental strains. Survival rate of pigs inoculated with 10(3.0)TCID(50) of SY18 Delta L7L was 25%, while all pigs inoculated with 10(3.0)TCID(50) of SY18 Delta L11L survived, and 50% inoculated with 10(6.0)TCID(50) SY18 Delta L11L survived. Discussion: The results indicate that L8L, L9R and L10L do not affect ASFV SY18 virulence, while the L7L and L11L are associated with virulence.
分类号：
- 相关文献
作者其他论文 更多>>
-
Appropriate stoichiometric ratios of dissolved organic carbon and nitrate can trigger a transition in nitrate removal in groundwater around plateau lakes, Southwest China
作者：Cui, Rongyang;Chen, Anqiang;Hu, Wanli;Fu, Bin;Cui, Rongyang;Liu, Gangcai;Cui, Rongyang;Zhang, Dan
关键词：Groundwater; Nitrate pollution; Dissolved organic carbon; Nitrate removal
-
DIA-Based Proteomic Analysis Reveals MYOZ2 as a Key Protein Affecting Muscle Growth and Development in Hybrid Sheep
作者：Zhang, Dan;Yue, Yaojing;Yuan, Chao;An, Xuejiao;Guo, Tingting;Chen, Bowen;Liu, Jianbin;Lu, Zengkui;Zhang, Dan;Yue, Yaojing;Yuan, Chao;An, Xuejiao;Guo, Tingting;Chen, Bowen;Liu, Jianbin;Lu, Zengkui
关键词：hybrid sheep; proteomic; MYOZ2; muscle; growth and development
-
Massively Parallel CRISPR-Cas9 Knockout Screening in Sheep Granulosa Cells for FSH Response Genes
作者：Liu, Zaixia;Dai, Lingli;Bao, Yanchun;Gu, Mingjuan;Shi, Caixia;Zhou, Le;Ma, Fengying;Na, Risu;Zhang, Wenguang;Liu, Zaixia;Dai, Lingli;Bao, Yanchun;Gu, Mingjuan;Guo, Lili;Zhou, Le;Ma, Fengying;Na, Risu;Zhang, Wenguang;Dai, Lingli;Sun, Tianhao;Liu, Yongbin;Fu, Shaoyin;He, Xiaolong;Wang, Yu;Guo, Lili;Zhang, Wenguang
关键词：granulosa cells; FSH; massively parallel CRISPR-Cas9 library; dose-dependent genes
-
Overexpression of a Fragaria vesca NAM, ATAF, and CUC (NAC) Transcription Factor Gene (FvNAC29) Increases Salt and Cold Tolerance in Arabidopsis thaliana
作者：Li, Wenhui;Li, Huiwen;Wei, Yangfan;Han, Jiaxin;Li, Xingguo;Zhang, Lihua;Han, Deguo;Wang, Yu
关键词：Fragaria vesca; FvNAC29; high-salinity stress; low-temperature stress
-
Enhanced assessment of regional impacts from wind erosion by integrating particle size
作者：Xu, Hui;Wang, Yu;Han, Tian;Li, Ruonan;Ma, Jinfeng;Ying, Lingxiao;Zheng, Hua;Xu, Hui;Wang, Yu;Li, Ruonan;Ma, Jinfeng;Ying, Lingxiao;Zheng, Hua;Han, Tian;Qiu, Xiao
关键词：Regional impact assessment; Soil loss; Sand and dust; Soil particle transportation; Particle size
-
Differential effects of winter cold stress on soil bacterial communities, metabolites, and physicochemical properties in two varieties of Tetrastigma hemsleyanum Diels & Gilg in reclaimed land
作者：Li, Xuqing;Ren, Xiaoxu;Ruan, Songlin;Yan, Jianli;Su, Yao;Zhou, Xiang;Wang, Yu;Li, Bin;Guo, Kai
关键词：Tetrastigma hemsleyanum; Diels & Gilg (TDG); winter cold stress; reclaimed land; 16S rRNA; soil bacterial communities; soil metabolites; soil properties
-
The Effects of Soybean-Tea Intercropping on the Photosynthesis Activity of Tea Seedlings Based on Canopy Spectral, Transcriptome and Metabolome Analyses
作者：Li, Xiaojiang;Wang, Shuangshuang;Sun, Litao;Shen, Jiazhi;Xu, Xiuxiu;Ding, Zhaotang;Xu, Yang;Mao, Yilin;Wang, Yu
关键词：intercropping; tea seedlings; canopy spectral; photosynthesis activity; light harvesting; photosystem
