文献类型： 外文期刊

第一作者： Lu, Pengyun

作者： Lu, Pengyun;Zhu, Yibin;Cai, Haiming;Liao, Shenquan;Qi, Nanshan;Lv, Minna;Lin, Xuhui;Song, Yongle;Chen, Xiangjie;Zhang, Jianfei;Li, Juan;Sun, Mingfei;Lu, Pengyun;Shen, Hanqin;Fang, Siyun;Wang, Dingai;Yan, Zhuanqiang

作者机构：

关键词： Pentatrichomonas hominis; Pig infection; Zoonotic potential; Prevalence; Genetic diversity

期刊名称：PARASITE （ 影响因子：2.4； 五年影响因子：2.7 ）

ISSN： 1252-607X

年卷期： 2025 年 32 卷

页码：

收录情况： SCI

摘要： Pentatrichomonas hominis is a protozoan parasite that infects the gastrointestinal tract of humans and mammals, causing abdominal pain and diarrhea. However, its presence in pigs and its potential as a pathogen causing diarrhea in piglets have not been well studied. This study aimed to investigate the prevalence of P. hominis in pigs and its potential for zoonotic transmission. A total of 406 pig fecal samples were collected from four pig farms located in Guangdong and Anhui Provinces. Fecal DNA extraction was carried out using a commercially available kit. A nested PCR methodology was employed to detect the presence of P. hominis infection. Samples that tested positive were subsequently subjected to sequencing, and the genetic characteristics of the 18S ribosomal RNA (rRNA) gene were analyzed. The overall infection rate of P. hominis was 34.98% (142/406), in Guangdong Province 28.47% (80/281), and in Anhui Province 49.60% (62/125). Among different age groups, suckling piglets had the highest infection rate at 40.24% (68/169). Genetic analysis of the P. hominis isolates showed that the PH-1 genotype was predominant and had a high degree of similarity to P. hominis sequences obtained from humans, cats, and pigs, indicating the potential for zoonotic transmission. The high infection rate and genetic diversity highlight the need for effective control measures in pig farming to reduce parasite transmission and zoonotic risk. Pentatrichomonas hominis est un parasite protozoaire qui infecte le tractus gastro-intestinal des humains et des mammif & egrave;res, provoquant douleurs abdominales et diarrh & eacute;es. Cependant, sa pr & eacute;sence chez les porcs et son potentiel pathog & egrave;ne causant des diarrh & eacute;es chez les porcelets n'ont pas & eacute;t & eacute; bien & eacute;tudi & eacute;s. Cette & eacute;tude visait & agrave; & eacute;valuer la pr & eacute;valence de P. hominis chez les porcs et son potentiel de transmission zoonotique. Au total, 406 & eacute;chantillons f & eacute;caux de porcs ont & eacute;t & eacute; collect & eacute;s dans quatre & eacute;levages porcins des provinces du Guangdong et de l'Anhui. L'extraction de l'ADN f & eacute;cal a & eacute;t & eacute; r & eacute;alis & eacute;e & agrave; l'aide d'un kit disponible dans le commerce. La m & eacute;thode de PCR nich & eacute;e a & eacute;t & eacute; utilis & eacute;e pour d & eacute;tecter la pr & eacute;sence d'une infection & agrave; P. hominis. Les & eacute;chantillons positifs ont ensuite & eacute;t & eacute; s & eacute;quenc & eacute;s et les caract & eacute;ristiques g & eacute;n & eacute;tiques du g & egrave;ne de l'ARN ribosomique (ARNr) 18S ont & eacute;t & eacute; analys & eacute;es. Le taux d'infection global de P. hominis & eacute;tait de 34,98 % (142/406), avec 28,47 % (80/281) dans la province du Guangdong et 49,60 % (62/125) dans la province de l'Anhui. Parmi les diff & eacute;rents groupes d'& acirc;ge, les porcelets allait & eacute;s pr & eacute;sentaient le taux d'infection le plus & eacute;lev & eacute;, soit 40,24 % (68/169). L'analyse g & eacute;n & eacute;tique des isolats de P. hominis a montr & eacute; que le g & eacute;notype PH-1 & eacute;tait pr & eacute;dominant et pr & eacute;sentait un degr & eacute; & eacute;lev & eacute; de similitude avec les s & eacute;quences de P. hominis obtenues chez l'homme, le chat et le porc, indiquant un potentiel de transmission zoonotique. Le taux d'infection & eacute;lev & eacute; et la diversit & eacute; g & eacute;n & eacute;tique soulignent la n & eacute;cessit & eacute; de mesures de contr & ocirc;le efficaces dans les & eacute;levages porcins pour r & eacute;duire la transmission des parasites et le risque zoonotique.

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