文献类型： 外文期刊

第一作者： Xia Xiao-Ming

作者： Xia Xiao-Ming;Wang Jin-Hua;Wang Kai-Yun;Liu Zhen-Long;Duan Hai-Ming

作者机构：

关键词： reversed phase high performance liquid chromatography; Rhizoctonta cerealis; ergosterol; lanosterol

期刊名称：CHINESE JOURNAL OF ANALYTICAL CHEMISTRY （ 影响因子：1.134； 五年影响因子：0.909 ）

ISSN： 0253-3820

年卷期： 2007 年 35 卷 2 期

页码：

收录情况： SCI

摘要： A determination method of ergosterol and lanosterol in mycelium of Rhizoctonia cerealis by reversed phase high performance liquid chromatography (RHPLC) was established. The sample was analyzed after broke up, centrifugating and saponifiing. The chromatographic column Diamonsil (TM) C-18 (250 mm x 4.6 min i.d., 5 mu m i.d) was used with 100% methanol as the mobile phase. The flow rate was 1 mL/min and the detected wavelength was set at 210 nm. The analytes were qualitated by retention time and quantitated by peak area using external standard method. The results show that the retention time of ergosterol and lanosterol is 21 and 27 min respectively. There are excellent linearity between peakarea and concentration of analytes in the concentration range of 0.125 - 12.5 mg/L for ergosterol and 0.1 - 10.0 mg/L for lanosterol, the correlation coefficient are 0.9993 and 0.9983 respectively. The average recoveries are 91.8% - 99.7%, the relative standard deviations are 2.8% - 7.8%. The detection limit of quantity is 1.0 ng and 0.8 ng respectively, the detection limit of concentration is 0.05 mg/L and 0.04 mg/L respectively. This method is simple, accurate and precise.

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