FERONIA receptor kinase-regulated reactive oxygen species mediate self-incompatibility in Brassica rapa

文献类型： 外文期刊

第一作者： Zhang, Lili

作者： Zhang, Lili;Huang, Jiabao;Su, Shiqi;Yang, Lin;Zhao, Huanhuan;Yu, Jianqiang;Wang, Jie;Hui, Jiyun;Song, Shanshan;Cao, Yanyan;Wang, Maoshuai;Zhang, Xiansheng;Duan, Qiaohong;Zhang, Lili;Huang, Jiabao;Su, Shiqi;Yang, Lin;Zhao, Huanhuan;Yu, Jianqiang;Wang, Jie;Hui, Jiyun;Song, Shanshan;Cao, Yanyan;Wang, Maoshuai;Duan, Qiaohong;Hao, Shiya;Wei, Xiaochun;Zhang, Xiaowei;Zhao, Yanyan;Wang, Zhiyong;Yuan, Yuxiang;Wu, Hen-Ming;Cheung, Alice Y.;Zeng, Weiqing;Zeng, Weiqing

作者机构：

期刊名称：CURRENT BIOLOGY （ 影响因子：10.834； 五年影响因子：11.713 ）

ISSN： 0960-9822

年卷期： 2021 年 31 卷 14 期

页码：

收录情况： SCI

摘要： Most plants in the Brassicaceae evolve self-incompatibility (SI) to avoid inbreeding and generate hybrid vigor. Self-pollen is recognized by the S-haplotype-specific interaction of the pollen ligand S-locus protein 11 (SP11) (also known as S-locus cysteine-rich protein [SCR]) and its stigma-specific S-locus receptor kinase (SRK). However, mechanistically much remains unknown about the signaling events that culminate in self pollen rejection. Here, we show that self-pollen triggers high levels of reactive oxygen species (ROS) in stigma papilla cells to mediate SI in heading Chinese cabbage (Brassica rapa L. ssp. pekinensis). We found that stigmatic ROS increased after self-pollination but decreased after compatible(CP)-pollination. Reducing stigmatic ROS by scavengers or suppressing the expression of respiratory burst oxidase homologs (Rbohs), which encode plant NADPH oxidases that produce ROS, both broke down SI. On the other hand, increasing the level of ROS inhibited the germination and penetration of compatible pollen on the stigma, mimicking an incompatible response. Furthermore, suppressing a B. rapa FERONIA (FER) receptor kinase homolog or Rac/ Rop guanosine triphosphatase (GTPase) signaling effectively reduced stigmatic ROS and interfered with SI. Our results suggest that FER-Rac/Rop signaling-regulated, NADPH oxidase-produced ROS is an essential SI response leading to self-pollen rejection.

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